紫外线诱导C3H 10T1/2小鼠细胞中哇巴因抗性突变
Induction of ouabain-resistant mutations in C3H 10T1/2 mouse cells by ultraviolet light.
作者信息
Chan G L, Little J B
出版信息
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3363-6. doi: 10.1073/pnas.75.7.3363.
Abstract
Ouabain-resistant mutants were induced in C3H mouse embryo 10T1/2 fibroblasts by exposure to ultraviolet light, thus making available an in vitro system for studying mutagenesis and oncogenic transformation in parallel. 86Rb uptake studies showed that biochemical mutants at the plasma membrane Na+,K+ transport ATPase (EC 3.6.1.3) locus were being selected for in this system. The optimal expression time for the mutants was found to depend on the dose of ultraviolet light, as was the induced mutation frequency. The ratio of transformation to mutation frequencies was found to be on the order of 10 for four different doses, suggesting that the target size in the cellular genome for transformation may be approximately 10 times the size of the Na+,K+ ATPase gene. We propose that both transformation and mutation induction can now be quantitatively studied in this single system.
摘要
通过紫外线照射在C3H小鼠胚胎10T1/2成纤维细胞中诱导出哇巴因抗性突变体，从而建立了一个可同时研究诱变和致癌转化的体外系统。86Rb摄取研究表明，该系统正在选择质膜Na +，K +转运ATP酶（EC 3.6.1.3）位点的生化突变体。发现突变体的最佳表达时间取决于紫外线剂量，诱导突变频率也是如此。对于四种不同剂量，转化频率与突变频率之比约为10，这表明细胞基因组中致癌转化的靶标大小可能约为Na +，K + ATP酶基因大小的10倍。我们认为现在可以在这个单一系统中对转化和诱变诱导进行定量研究。
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