Czjzek Mirjam, Bravman Tsafrir, Henrissat Bernard, Shoham Yuval
Architecture et Fonction des Macromolécules Biologiques, CNRS-Université de Provence, Université d'Aix-Marseille II, IBSM, 31 Chemin Joseph-Aiguier, 13402 Marseille CEDEX 20, France.
Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):583-5. doi: 10.1107/S0907444904001088. Epub 2004 Feb 25.
beta-D-Xylosidases (EC 3.2.1.37) are hemicellulases that hydrolyze short xylooligosaccharides into single xylose units. In this study, the crystallization and preliminary X-ray analysis of the beta-D-xylosidase (XynB1) from Geobacillus stearothermophilus T-6, a family 39 glycoside hydrolase, are described. XynB1 is a tetrameric protein consisting of four identical subunits of 503 amino acids and with a calculated molecular weight of 58 001 Da. Both the native and the selenomethionine-containing XynB1 were crystallized by the hanging-drop vapour-diffusion method and the crystals were found to belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 92.7, b = 165.7, c = 311.0 A. The native crystals diffracted X-rays to a resolution of 2.1 A.
β-D-木糖苷酶（EC 3.2.1.37）是一种半纤维素酶，可将短木寡糖水解为单个木糖单元。在本研究中，描述了嗜热栖热放线菌T-6来源的β-D-木糖苷酶（XynB1，一种39家族糖苷水解酶）的结晶及初步X射线分析。XynB1是一种四聚体蛋白，由四个相同的503个氨基酸亚基组成，计算分子量为58001 Da。通过悬滴气相扩散法使天然型和含硒代甲硫氨酸的XynB1均结晶，发现晶体属于空间群P2(1)2(1)2(1)，晶胞参数a = 92.7、b = 165.7、c = 311.0 Å。天然晶体对X射线的衍射分辨率为2.1 Å。
