属于糖苷水解酶家族5的新型里氏木霉木聚糖酶IV的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5.
作者信息
Parkkinen Tarja, Hakulinen Nina, Tenkanen Maija, Siika-aho Matti, Rouvinen Juha
机构信息
Department of Chemistry, University of Joensuu, PO Box 111, FIN-80101 Joensuu, Finland.
出版信息
Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):542-4. doi: 10.1107/S0907444903029123. Epub 2004 Feb 25.
Xylanase IV (XYN IV) is a new recently characterized xylanase from Trichoderma reesei. It is able to degrade several different xylans, mainly producing xylose. XYN IV has been crystallized by the hanging-drop vapour-diffusion method, using PEG 6000 as a precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 86.3, b = 137.5, c = 196.1 A, alpha = beta = gamma = 90 degrees. Assuming a molecular weight of 50.3 kDa, the V(M) values indicate there to be four XYN IV monomers in an asymmetric unit and the solvent content of the crystals to be 57%. Based on dynamic light-scattering measurements, XYN IV is a dimer in solution. A native data set to 2.8 A resolution has been collected at a home laboratory and a data set to 2.2 A resolution has been collected using synchrotron radiation.
木聚糖酶IV（XYN IV）是一种最近从里氏木霉中鉴定出的新型木聚糖酶。它能够降解几种不同的木聚糖，主要产生木糖。XYN IV已通过悬滴气相扩散法结晶，使用聚乙二醇6000作为沉淀剂。晶体属于正交晶系空间群P2(1)2(1)2(1)，晶胞参数a = 86.3，b = 137.5，c = 196.1 Å，α = β = γ = 90°。假设分子量为50.3 kDa，V(M)值表明一个不对称单元中有四个XYN IV单体，晶体的溶剂含量为57%。基于动态光散射测量，XYN IV在溶液中是二聚体。已在国内实验室收集了分辨率为2.8 Å的天然数据集，并使用同步辐射收集了分辨率为2.2 Å的数据集。
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