Lukatsky D B, Frenkel Daan
FOM Institute for Atomic and Molecular Physics, Kruislaan 407, 1098 SJ Amsterdam, The Netherlands.
Phys Rev Lett. 2004 Feb 13;92(6):068302. doi: 10.1103/PhysRevLett.92.068302. Epub 2004 Feb 10.
We propose a model that can account for the experimentally observed phase behavior of DNA-nanoparticle assemblies [J. Am. Chem. Soc. 125, 1643 (2003)]; Science 289, 1757 (2000)]]. The binding of DNA-coated nanoparticles by dissolved DNA linkers can be described by exploiting an analogy with quantum particles obeying fractional statistics. In accordance with experimental findings, we predict that the phase-separation temperature of the nanocolloids increases with the DNA coverage of the colloidal surface. Upon the addition of salt, the demixing temperature increases logarithmically with the salt concentration. Our analysis suggests an experimental strategy to map microscopic DNA sequences onto the macroscopic phase behavior of the DNA-nanoparticle solutions. Such an approach should enhance the efficiency of methods to detect (single) mutations in specific DNA sequences.
我们提出了一个模型，该模型能够解释实验观察到的DNA-纳米颗粒组装体的相行为[《美国化学会志》125, 1643 (2003)]；《科学》289, 1757 (2000)]。溶解的DNA连接体与DNA包覆的纳米颗粒之间的结合，可以通过与遵循分数统计的量子粒子进行类比来描述。根据实验结果，我们预测纳米胶体的相分离温度会随着胶体表面DNA覆盖率的增加而升高。加入盐后，分层温度会随着盐浓度呈对数增加。我们的分析提出了一种实验策略，可将微观DNA序列映射到DNA-纳米颗粒溶液的宏观相行为上。这种方法应能提高检测特定DNA序列中（单个）突变的方法的效率。
