Brinsmade Shaun R, Escalante-Semerena Jorge C
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53726, USA.
J Bacteriol. 2004 Mar;186(6):1890-2. doi: 10.1128/JB.186.6.1890-1892.2004.
The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the product of the reaction. The EutD protein restored growth of an S. enterica pta strain on acetate as the source of carbon and energy.
肠炎沙门氏菌的EutD蛋白与磷酸转乙酰酶(Pta)的催化结构域同源。EutD酶的类Pta活性水平与其他Pta酶相比具有优势。高压液相色谱和质谱分析证实乙酰辅酶A是该反应的产物。EutD蛋白恢复了以乙酸盐作为碳源和能源的肠炎沙门氏菌pta菌株的生长。