Ortega-Moreno J, Caballero-Gómez J M
Department of Animal Pathology, University of Zaragoza, Spain.
Eur J Obstet Gynecol Reprod Biol. 1992 Jul 3;45(2):139-45. doi: 10.1016/0028-2243(92)90230-v.
In the hope of identifying suitable material for reconstructive tubal and uterine surgery, the authors have replaced a portion of the uterine horns of two groups of Wistar rats (n = 10) with normal (VAS group) or with denervated and devascularized jejunal grafts (NVAS group). All grafted horns stayed patent. No pregnancy was observed in the uterine horns operated on. The light microscopic appearance of the grafts was studied and compared by ANOVA. Three months after surgery in the VAS group, the number (51.4 +/- 9.5) and height (309.8 +/- 44.7 microns, P less than 0.05) of the villus, the Lieberkühn cripts height (156.9 +/- 34.9 microns) and the number of goblet cells per cript (7.2 +/- 1.2, P less than 0.05) had decreased in relation to their corresponding control jejunal fragments (57.7 +/- 5.4; 345.1 +/- 18.0 microns; 192.2 +/- 40.8 micron; 12.4 +/- 1.9, respectively). Three months after surgery, the intestinal structure was maintained in the anastomosis areas of the NVAS group, therefore the mucus production of the jejunal-graft mucosa was not completely eliminated. No villus or crypts were observed, but a monostratified cubic-cell-type epithelium at the NVAS jejunal graft mid-portion, along 58.1 +/- 12.9% of the total length of the intestinal graft. Under this epithelium a connective tissue, like a non-glandular submucosa, was apparent.
为了寻找适合输卵管和子宫重建手术的材料，作者用正常空肠移植物（VAS组）或去神经和去血管化空肠移植物（NVAS组）替换了两组Wistar大鼠（n = 10）的部分子宫角。所有移植的子宫角均保持通畅。未观察到手术操作的子宫角中有妊娠情况。通过方差分析研究并比较了移植物的光镜外观。在VAS组手术三个月后，绒毛数量（51.4±9.5）和高度（309.8±44.7微米，P<0.05）、利伯孔隐窝高度（156.9±34.9微米）以及每个隐窝杯状细胞数量（7.2±1.2，P<0.05）相较于相应的对照空肠片段（分别为57.7±5.4；345.1±18.0微米；192.2±40.8微米；12.4±1.9）有所减少。手术三个月后，NVAS组吻合区域的肠道结构得以维持，因此空肠移植物黏膜的黏液分泌并未完全消除。未观察到绒毛或隐窝，但在NVAS空肠移植物中部，沿肠道移植物总长度的58.1±12.9%可见单层立方细胞型上皮。在此上皮下方，有一层类似非腺性黏膜下层的结缔组织。
