泛素连接酶Nedd4对人1型T细胞白血病病毒（HTLV-1）出芽的调控

Regulation of human T-cell leukemia virus type 1 (HTLV-1) budding by ubiquitin ligase Nedd4.

作者信息

Sakurai Akira, Yasuda Jiro, Takano Hiroko, Tanaka Yuetsu, Hatakeyama Masanori, Shida Hisatoshi

机构信息

Division of Molecular Virology, Institute for Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo 060-0815, Japan.

出版信息

Microbes Infect. 2004 Feb;6(2):150-6. doi: 10.1016/j.micinf.2003.10.011.

DOI:10.1016/j.micinf.2003.10.011

PMID:14998512

Abstract

The Gag protein of human T-cell leukemia virus type 1 (HTLV-1) contains the conserved sequences PPxY and PTAP, which are putative viral motifs required for budding (L-domain motifs). We show here that the PPxY motif, but not the PTAP motif, is essential for HTLV-1 virion budding from the plasma membrane. In addition, we show that overexpression of Nedd4 enhances HTLV-1 budding and that Nedd4 interacts with Gag via its WW domain. The HECT domain of Nedd4 is also required for budding. These results indicate that Nedd4 or a Nedd4-related ubiquitin ligase plays a critical role in HTLV-1 budding.

摘要

人类嗜T淋巴细胞病毒1型（HTLV-1）的Gag蛋白含有保守序列PPxY和PTAP，它们是出芽所需的假定病毒基序（L结构域基序）。我们在此表明，PPxY基序而非PTAP基序对于HTLV-1病毒粒子从质膜出芽至关重要。此外，我们表明Nedd4的过表达增强了HTLV-1出芽，并且Nedd4通过其WW结构域与Gag相互作用。Nedd4的HECT结构域对于出芽也是必需的。这些结果表明，Nedd4或与Nedd4相关的泛素连接酶在HTLV-1出芽中起关键作用。