烟曲霉基因组解析：对包含硝酸盐同化基因簇的922 kb区域的分析

Insight into the genome of Aspergillus fumigatus: analysis of a 922 kb region encompassing the nitrate assimilation gene cluster.

作者信息

Pain Arnab, Woodward John, Quail Michael A, Anderson Michael J, Clark Richard, Collins Matthew, Fosker Nigel, Fraser Audrey, Harris David, Larke Natasha, Murphy Lee, Humphray Sean, O'Neil Susan, Pertea Mihaela, Price Claire, Rabbinowitsch Ester, Rajandream Marie-Adele, Salzberg Steven, Saunders David, Seeger Kathy, Sharp Sarah, Warren Tim, Denning David W, Barrell Bart, Hall Neil

机构信息

The Pathogen Sequencing Unit, The Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge CB10 1SA, UK.

出版信息

Fungal Genet Biol. 2004 Apr;41(4):443-53. doi: 10.1016/j.fgb.2003.12.003.

DOI:10.1016/j.fgb.2003.12.003

PMID:14998527

Abstract

Aspergillus fumigatus is the most ubiquitous opportunistic filamentous fungal pathogen of human. As an initial step toward sequencing the entire genome of A. fumigatus, which is estimated to be approximately 30 Mb in size, we have sequenced a 922 kb region, contained within 16 overlapping bacterial artificial chromosome (BAC) clones. Fifty-four percent of the DNA is predicted to be coding with 341 putative protein coding genes. Functional classification of the proteins showed the presence of a higher proportion of enzymes and membrane transporters when compared to those of Saccharomyces cerevisiae. In addition to the nitrate assimilation gene cluster, the quinate utilisation gene cluster is also present on this 922 kb genomic sequence. We observed large scale synteny between A. fumigatus and Aspergillus nidulans by comparing this sequence to the A. nidulans genetic map of linkage group VIII.

摘要

烟曲霉是人类最常见的机会性丝状真菌病原体。作为对估计大小约为30 Mb的烟曲霉全基因组进行测序的第一步，我们对包含在16个重叠细菌人工染色体（BAC）克隆中的922 kb区域进行了测序。预计54%的DNA可编码，共有341个推定的蛋白质编码基因。与酿酒酵母相比，这些蛋白质的功能分类显示酶和膜转运蛋白的比例更高。除了硝酸盐同化基因簇外，922 kb的基因组序列上还存在奎尼酸利用基因簇。通过将该序列与构巢曲霉VIII连锁群的遗传图谱进行比较，我们观察到烟曲霉和构巢曲霉之间存在大规模的共线性。