Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8673, Japan.
Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, 292-0818, Japan.
Commun Biol. 2024 Mar 14;7(1):274. doi: 10.1038/s42003-024-05902-6.
Aspergillus fumigatus is a pathogenic fungus with a global distribution. The emergence of azole-resistant A. fumigatus (ARAf) other than the TR-mutants is a problem in Japan. Additionally, the genetic diversity of A. fumigatus strains in Japan remains relatively unknown. Here we show the diversity in the A. fumigatus strains isolated in Japan as well as the complexity in the global distribution of the pathogenic strains. First, we analyzed the genome sequences of 171 strains from Japan as well as the antifungal susceptibility of these strains. Next, we conducted a population analysis of 876 strains by combining the available genomic data for strains isolated worldwide, which were grouped in six clusters. Finally, a genome-wide association study identified the genomic loci associated with ARAf strains, but not the TR-mutants. These results highlight the complexity of the genomic mechanism underlying the emergence of ARAf strains other than the TR-mutants.
烟曲霉是一种具有全球分布的致病性真菌。除了 TR 突变株之外，唑类耐药的烟曲霉（ARAf）的出现是日本的一个问题。此外，日本烟曲霉菌株的遗传多样性仍然相对未知。在这里，我们展示了在日本分离的烟曲霉菌株的多样性以及致病菌株在全球的分布复杂性。首先，我们分析了来自日本的 171 株菌株的基因组序列以及这些菌株的抗真菌药敏性。接下来，我们通过结合来自世界各地分离的菌株的可用基因组数据对 876 株菌株进行了种群分析，这些菌株被分为六个簇。最后，全基因组关联研究确定了与 ARAf 菌株相关的基因组位点，但与 TR 突变株无关。这些结果突出了除 TR 突变株之外，ARAf 菌株出现的基因组机制的复杂性。
