稻瘟病菌和粗糙脉孢菌中的微共线性区域。

Regions of microsynteny in Magnaporthe grisea and Neurospora crassa.

作者信息

Hamer L, Pan H, Adachi K, Orbach M J, Page A, Ramamurthy L, Woessner J P

机构信息

Paradigm Genetics, Inc., 108 Alexander Drive, Research Triangle Park, NC 27709, USA.

出版信息

Fungal Genet Biol. 2001 Jul;33(2):137-43. doi: 10.1006/fgbi.2001.1286.

DOI:10.1006/fgbi.2001.1286

PMID:11456466

Abstract

A bacterial artificial chromosome (BAC) clone containing 110,467 bp of genomic DNA from Magnaporthe grisea was sequenced, annotated, and compared to the genomes of Neurospora crassa, Candida albicans, and Saccharomyces cerevisiae. Twenty-six open reading frames (ORFs), involved in multiple biochemical pathways, were identified in the BAC sequence. A region of 53 kb, containing 18 of the 26 ORFs, was found to be syntenic to a portion of the N. crassa genome. Subregions of complete colinearity as well as interrupted colinearity were present. No synteny was evident with either C. albicans or S. cerevisiae. The identification of syntenic regions containing highly conserved genes across two genera that have been evolutionarily separated for approximately 200 million years elicits many biological questions as to the function and identity of these genes.

摘要

对一个包含来自稻瘟病菌110467 bp基因组DNA的细菌人工染色体（BAC）克隆进行了测序、注释，并与粗糙脉孢菌、白色念珠菌和酿酒酵母的基因组进行了比较。在BAC序列中鉴定出26个参与多种生化途径的开放阅读框（ORF）。发现一个53 kb的区域，包含26个ORF中的18个，与粗糙脉孢菌基因组的一部分同线。存在完全共线性以及间断共线性的亚区域。与白色念珠菌或酿酒酵母均未发现明显的同线性。在进化上已经分离了约2亿年的两个属中鉴定出包含高度保守基因的同线区域，引发了许多关于这些基因的功能和特性的生物学问题。