Shannon Simon K, Barany George
Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, MN 55455, USA.
J Comb Chem. 2004 Mar-Apr;6(2):165-70. doi: 10.1021/cc034033x.
A simple and rapid method to achieve colorimetric monitoring of resin-bound aldehydes, based on ambient temperature reaction with 2,4-dinitrophenylhydrazine (DNPH) in the presence of dilute acid, has been developed as an adjunct to solid-phase organic synthesis and combinatorial chemistry. By this test, the presence of aldehydes is indicated by a red to dark-orange appearance, within a minute. Alternatively, resins that are free of aldehydes or in which aldehyde functions have reacted completely retain their original color. The DNPH test was demonstrated for poly(ethylene glycol)-polystyrene (PEG-PS), aminomethyl polystyrene (AMP), cross-linked ethoxylate acrylate resin (CLEAR), and acryloylated O,O'-bis(2-aminopropyl)poly(ethylene glycol) (PEGA) supports and gave results visible to the naked eye at levels as low as 18 micromol of aldehyde per gram of resin.
已开发出一种简单快速的方法，用于比色监测树脂结合的醛，该方法基于在稀酸存在下与2,4-二硝基苯肼（DNPH）在环境温度下的反应，作为固相有机合成和组合化学的辅助手段。通过此测试，醛的存在在一分钟内表现为从红色到深橙色。另外，不含醛或醛官能团已完全反应的树脂会保持其原始颜色。对聚（乙二醇）-聚苯乙烯（PEG-PS）、氨甲基聚苯乙烯（AMP）、交联乙氧基化丙烯酸酯树脂（CLEAR）和丙烯酰化的O,O'-双（2-氨丙基）聚（乙二醇）（PEGA）载体进行了DNPH测试，结果表明，每克树脂中醛含量低至18微摩尔时，肉眼即可看到测试结果。
