Kates S A, McGuinness B F, Blackburn C, Griffin G W, Solé N A, Barany G, Albericio F
PerSeptive Biosystems, Inc., Framingham, MA 01701, USA.
Biopolymers. 1998;47(5):365-80. doi: 10.1002/(SICI)1097-0282(1998)47:5<365::AID-BIP4>3.0.CO;2-8.
The choice of a polymeric support is a key factor for the success of solid-phase methods for syntheses of organic compounds and biomolecules such as peptides and oligonucleotides. Classical Merrifield solid-phase peptide synthesis (SPPS), performed on low cross-linked hydrophobic polystyrene (PS) beads, sometimes suffers from sequence-dependent coupling difficulties. The concept of incorporating polyethylene glycol (PEG) into supports for solid-phase synthesis represents a successful approach to alleviating such problems. Previous reports from our laboratories have shown the advantages of "low-load" PEG-PS (0.15-0.25 mmol/g) for SPPS. Herein, we demonstrate that the beneficial aspects of the PEG-PS concept can be extended with resins that have higher loadings (0.3-0.5 mmol/g).
对于有机化合物以及肽和寡核苷酸等生物分子的固相合成方法而言，聚合物载体的选择是成功的关键因素。在低交联疏水聚苯乙烯（PS）珠粒上进行的经典梅里菲尔德固相肽合成（SPPS）有时会遇到序列依赖性偶联困难。将聚乙二醇（PEG）引入固相合成载体的概念是缓解此类问题的一种成功方法。我们实验室之前的报告已经表明了“低负载量”PEG-PS（0.15 - 0.25 mmol/g）用于SPPS的优势。在此，我们证明PEG-PS概念的有益方面可以扩展到具有更高负载量（0.3 - 0.5 mmol/g）的树脂上。
