Preziosi R, Sarli G, Paltrinieri M
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Settore Patologia Generale ed Anatomia Patologica, via Tolara di Sopra 50, 40064 Ozzano Emilia, Bologna, Italy.
J Comp Pathol. 2004 Feb-Apr;130(2-3):143-51. doi: 10.1016/j.jcpa.2003.10.003.
The biological behaviour of canine cutaneous mast cell tumours (MCTs) is highly variable. The aim of this study was to investigate whether intratumoral microvessel density (IMVD), as an indicator of angiogenesis, was predictive of biological behaviour in MCTs of canine skin, and to relate this parameter to histological grade and other histomorphological features. IMVD was evaluated by immunohistochemical labelling of the endothelial marker factor VIII (Von Willebrand's factor)-related antigen in 32 primary cutaneous MCTs. Computer image analysis was used to measure IMVD, expressed as the number of microvessels per square millimetre. Cases were assigned to low and high vessel density groups, the median value (14.1) being used as the "cut-off". Kaplan-Meier-estimated survival curves of the groups revealed that IMVD was significantly associated with survival time (P=0.046). One-way analysis of variance, performed to compare the IMVD values of tumours of different histological grades, showed a significant difference between grades I and III (P=0.0002). There was a significant difference in IMVD between moderately invasive and highly invasive tumours (P=0.002) and between tumours with different numbers of mitoses (P=0.0046). In multivariate analysis, performed to test the combined influence on survival of each parameter, only the mitotic index and IMVD were independent prognostic variables (P=0.00019). The relation of angiogenesis to biological behaviour and histological parameters of malignancy of cutaneous MCTs was consistent with previous findings in human and animal tumours.
犬皮肤肥大细胞瘤（MCTs）的生物学行为具有高度变异性。本研究的目的是调查作为血管生成指标的肿瘤内微血管密度（IMVD）是否可预测犬皮肤MCTs的生物学行为，并将该参数与组织学分级及其他组织形态学特征相关联。通过对32例原发性皮肤MCTs的内皮标志物因子VIII（血管性血友病因子）相关抗原进行免疫组织化学标记来评估IMVD。使用计算机图像分析测量IMVD，以每平方毫米微血管数量表示。病例被分为低血管密度组和高血管密度组，中位数（14.1）用作“临界值”。两组的Kaplan-Meier估计生存曲线显示，IMVD与生存时间显著相关（P = 0.046）。进行单因素方差分析以比较不同组织学分级肿瘤的IMVD值，结果显示I级和III级之间存在显著差异（P = 0.0002）。中度侵袭性和高度侵袭性肿瘤之间的IMVD存在显著差异（P = 0.002），不同有丝分裂数的肿瘤之间也存在显著差异（P = 0.0046）。在多因素分析中，为测试每个参数对生存的综合影响，只有有丝分裂指数和IMVD是独立的预后变量（P = 0.00019）。血管生成与皮肤MCTs的生物学行为及恶性组织学参数之间的关系与先前在人类和动物肿瘤中的发现一致。
