采用带紫外检测的高效液相色谱法对研究用抗肿瘤药物5,10-二去氮-5,6,7,8-四氢叶酸进行生物分析。

Bioanalysis of the investigational anti-tumour drug 5,10-dideaza-5,6,7,8-tetrahydrofolic acid by high-performance liquid chromatography with ultraviolet detection.

作者信息

van Tellingen O, Sips J H, Beijnen J H, Schornagel J H, Nooyen W J

机构信息

Department of Clinical Chemistry, Netherlands Cancer Institute, Amsterdam.

出版信息

J Chromatogr. 1992 Apr 15;576(1):158-62. doi: 10.1016/0378-4347(92)80188-v.

DOI:10.1016/0378-4347(92)80188-v

PMID:1500451

Abstract

A high-performance liquid chromatographic (HPLC) method with ultraviolet detection at 278 nm is presented for the determination of 5,10-dideaza-5,6,7,8-tetrahydrofolic acid in plasma. Sample pretreatment was achieved by using cation-exchange solid-phase extraction columns with methotrexate as internal standard. Chromatographic separation was based on ion-pair HPLC with 1-octanesulphonic acid as the ion-pairing compound. The detection limit was 10 ng/ml using an 500-microliters sample volume. The assay was linear from the detection limit up to 5000 ng/ml with good reproducibility. The applicability of the assay was demonstrated in a study in the rat.

摘要

本文介绍了一种采用278 nm紫外检测的高效液相色谱（HPLC）法，用于测定血浆中的5,10-二去氮-5,6,7,8-四氢叶酸。以甲氨蝶呤为内标，采用阳离子交换固相萃取柱进行样品预处理。色谱分离基于离子对HPLC，使用1-辛烷磺酸作为离子对化合物。使用500微升进样体积时，检测限为10 ng/ml。该测定法在检测限至5000 ng/ml范围内呈线性，具有良好的重现性。该测定法的适用性在大鼠研究中得到了验证。