Proksch B, Blanz J, Zeller K P, Ehninger G
Medizinische Klinik and Poliklinik, Universität Tübingen, Germany.
J Chromatogr B Biomed Appl. 1994 Aug 19;658(2):349-53. doi: 10.1016/0378-4347(94)00235-5.
A high-performance liquid chromatographic method using ion-pairing chromatography on reversed-phase C18 material with a mobile phase of acetonitrile-water (19:81, v/v) containing 5 mM 1-pentanesulfonic acid was developed for the detection and separation of the anthrapyrazole CI-941 (I) and its metabolites. After sample clean-up with solid-phase extraction, I and its metabolites were measurable at a wavelength of 491 nm. A detection limit of 5 ng/ml was achievable for I. The dicarboxylic acid derivative and the isomers of the monocarboxylic acid derivative could be separated. Application of the method to a human pharmacokinetic study showed two and four metabolites of I in serum and urine respectively.
