Rivat C, Sertillanges P, Patin E, Stoltz J F
Unité INSERM U 284, Vandoeuvre-les-Nancy, France.
J Chromatogr. 1992 Apr 15;576(1):71-7. doi: 10.1016/0378-4347(92)80176-q.
A rapid, simple and convenient method is described for the isolation, on a pilot scale, of pure and functional human transferrin from an unexploited by-product of chromatographic fractionation of plasma. In a single chromatographic step on DEAE-Spherodex, 97% pure transferrin was obtained in 75% yield. A virus inactivation treatment was included in the preparative process in order to guarantee the safety of the final product, which could be used in culture media.
本文描述了一种快速、简单且便捷的方法，可从中规模分离血浆色谱分离未开发的副产物中获得纯的且具有功能的人转铁蛋白。在DEAE-Spherodex上进行单次色谱步骤，可获得纯度为97%、产率为75%的转铁蛋白。在制备过程中包含病毒灭活处理，以确保最终产品的安全性，该产品可用于培养基。
