高纯度人转铁蛋白的快速制备
Rapid preparation of highly purified human transferrin.
作者信息
Cook N D, Simpson R J, Osterloh K, Peters T J
出版信息
Anal Biochem. 1985 Sep;149(2):349-53. doi: 10.1016/0003-2697(85)90581-0.
PMID:4073492
Abstract
A rapid, two-step purification for human transferrin using DEAE-Sephacel chromatography followed by phenyl-boronate affinity chromatography is described. The method gives a 70% yield and isolates transferrin of over 97% purity which is hemopexin-free. The remaining protein is due to a single contaminant which may be the glycosylated form of albumin.
摘要
本文描述了一种使用DEAE-琼脂糖凝胶离子交换层析继以苯基硼酸亲和层析对人转铁蛋白进行快速两步纯化的方法。该方法产率为70%，分离得到的转铁蛋白纯度超过97%，且不含血红素结合蛋白。剩余的蛋白质是由于单一污染物导致的，该污染物可能是白蛋白的糖基化形式。
Zotero 插件