Rapid preparation of highly purified human transferrin.
作者信息
Cook N D, Simpson R J, Osterloh K, Peters T J
出版信息
Anal Biochem. 1985 Sep;149(2):349-53. doi: 10.1016/0003-2697(85)90581-0.
PMID:4073492
Abstract
A rapid, two-step purification for human transferrin using DEAE-Sephacel chromatography followed by phenyl-boronate affinity chromatography is described. The method gives a 70% yield and isolates transferrin of over 97% purity which is hemopexin-free. The remaining protein is due to a single contaminant which may be the glycosylated form of albumin.
摘要
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