Chen Lin, Ren Jicun, Bi Rui, Chen Di
College of Chemistry and Chemical Engineering, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P.R. China.
Electrophoresis. 2004 Mar;25(6):914-21. doi: 10.1002/elps.200305766.
Simple sealing methods for poly(dimethylsiloxane) (PDMS)/glass-based capillary electrophoresis (CE) microchips by UV irradiation are described. Further, we examined the possibility to modify the inner surface of separation channels, using polymethylacrylamide (PDMA) as a dynamic coating reagent. The surface properties of native PDMS, UV-irradiated PDMS, and PDMA-coated PDMS were systematically studied by atomic force microscopy (AFM), infrared absorption by attenuated total reflection infrared (ATR-IR) spectroscopy, and contact angle measurement. We found that PDMA forms a stable coating on PDMS and glass surfaces, eliminating the nonhomogeneous electroosmotic flow (EOF) in channels on PDMS/glass microchips, and improving the hydrophilicity of PDMS surfaces. Mixtures of flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), and fluorescein were separated in 35 s using PDMA-coated PDMS/glass microchips. A high efficiency of theoretical plates with at least 1365 (105 000 N/m) and a good reproducibility with relative standard deviations (RSD) below 4% in five successive separations were achieved.
本文描述了通过紫外线照射对聚二甲基硅氧烷（PDMS）/玻璃基毛细管电泳（CE）微芯片进行简单密封的方法。此外，我们研究了使用聚甲基丙烯酰胺（PDMA）作为动态涂层试剂来修饰分离通道内表面的可能性。通过原子力显微镜（AFM）、衰减全反射红外（ATR-IR）光谱法的红外吸收以及接触角测量，系统地研究了天然PDMS、紫外线照射的PDMS和PDMA涂层的PDMS的表面性质。我们发现，PDMA在PDMS和玻璃表面形成稳定的涂层，消除了PDMS/玻璃微芯片通道中不均匀的电渗流（EOF），并提高了PDMS表面的亲水性。使用PDMA涂层的PDMS/玻璃微芯片，在35秒内分离了黄素单核苷酸（FMN）、黄素腺嘌呤二核苷酸（FAD）和荧光素的混合物。在五次连续分离中，实现了至少1365（105000 N/m）的高理论塔板数和低于4%的相对标准偏差（RSD）的良好重现性。
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