School of Chemistry and Environmental Science, Henan Key Laboratory for Environmental Pollution Control, Henan Normal University, Jianshe Road, Xinxiang, Henan 453007, China.
Anal Chim Acta. 2010 Jan 18;658(1):75-80. doi: 10.1016/j.aca.2009.10.052. Epub 2009 Oct 29.
A novel covalent strategy was developed to modify the poly(dimethylsiloxane) (PDMS) surface. Briefly, dextran was selectively oxidized to aldehyde groups with sodium periodate and subsequently grafted onto amine-functionalized PDMS surface via Schiff base reaction. As expected, the coated PDMS surface efficiently prevented the biomolecules from adsorption. Electro-osmotic flow (EOF) was successfully suppressed compared with that on the native PDMS microchip. Moreover, the stability of EOF was greatly enhanced and the hydrophilicity of PDMS surface was also improved. To apply thus-coated microchip, the separation of peptides, protein and neurotransmitters was investigated in detail. For comparison, these analytes were also measured on the native PDMS microchips. The results demonstrated that these analytes were efficiently separated and detected on the coated PDMS microchips. Furthermore, the relative standard deviations of their migration times for run-to-run, day-to-day, and chip-to-chip reproducibilities were in the range of 0.6-2.7%. In addition, the coated PDMS microchips showed good stability within 1 month.
一种新的共价策略被开发用于修饰聚二甲基硅氧烷（PDMS）表面。简要地说，葡聚糖被高碘酸钠选择性氧化为醛基，然后通过席夫碱反应接枝到胺功能化的 PDMS 表面上。正如预期的那样，涂覆的 PDMS 表面有效地防止了生物分子的吸附。与天然 PDMS 微芯片相比，电泳流（EOF）得到了成功抑制。此外，EOF 的稳定性大大提高，PDMS 表面的亲水性也得到了改善。为了应用这种涂覆的微芯片，详细研究了肽、蛋白质和神经递质的分离。为了比较，这些分析物也在天然 PDMS 微芯片上进行了测量。结果表明，这些分析物在涂覆的 PDMS 微芯片上得到了有效分离和检测。此外，它们的迁移时间的相对标准偏差在 0.6-2.7%的范围内，具有运行间、日间和芯片间的重现性。此外，在 1 个月内，涂覆的 PDMS 微芯片表现出良好的稳定性。
