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新凶手弗朗西斯菌（U112）脂多糖O抗原的特性分析

Characterization of the lipopolysaccharide O-antigen of Francisella novicida (U112).

作者信息

Vinogradov Evgeny, Conlan Wayne J, Gunn John S, Perry Malcolm B

机构信息

Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada K1A OR6.

出版信息

Carbohydr Res. 2004 Feb 25;339(3):649-54. doi: 10.1016/j.carres.2003.12.013.

DOI:10.1016/j.carres.2003.12.013

PMID:15013402

Abstract

Francisella novicida (U112), a close relative of the highly virulent bacterium F. tularensis, was shown to produce a lipopolysaccharide in which the antigenic O-polysaccharide component was found by chemical, 1H and 13C NMR and MS analyses to be an unbranched neutral linear polymer of a repeating tetrasaccharide unit composed of 2-acetamido-2-deoxy-D-galacturonamide (D-GalNAcAN) and 2,4-diacetamido-2,4,6-trideoxy-D-glucose (D-Qui2NAc4NAc, di-N-acetylbacillosamine) residues (3:1) and had the structure: -->4)-alpha-D-GalNAcAN-(1-->4)-alpha-D-GalNAcAN-(1-->4)-alpha-D-GalNAcAN-(1-->3)-alpha-D-QuiNAc4NAc-(1-->. With polyclonal murine antibody, the F. novicida O-antigen did not show serological cross-reactivity with the O-antigen of F. tularensis despite the occurrence of a common -->4)-D-GalpNAcAN-(1-->4)-alpha-D-GalpNAcAN-(1--> disaccharide unit in their respective O-antigens. Thus, O-PS serology offers a practical way to distinguish between the two Francisella species.

摘要

新凶手弗朗西斯菌（U112）是高毒力细菌土拉弗朗西斯菌的近亲，已证明其产生一种脂多糖，通过化学分析、1H和13C核磁共振以及质谱分析发现，其中的抗原性O-多糖成分是由2-乙酰氨基-2-脱氧-D-半乳糖醛酰胺（D-GalNAcAN）和2,4-二乙酰氨基-2,4,6-三脱氧-D-葡萄糖（D-Qui2NAc4NAc，二-N-乙酰基芽孢胺）残基（3:1）组成的重复四糖单元的无分支中性线性聚合物，其结构为：→4)-α-D-GalNAcAN-(1→4)-α-D-GalNAcAN-(1→4)-α-D-GalNAcAN-(1→3)-α-D-QuiNAc4NAc-(1→。使用多克隆鼠抗体时，尽管新凶手弗朗西斯菌和土拉弗朗西斯菌各自的O抗原中存在共同的→4)-D-GalpNAcAN-(1→4)-α-D-GalpNAcAN-(1→二糖单元，但新凶手弗朗西斯菌的O抗原与土拉弗朗西斯菌的O抗原未表现出血清学交叉反应。因此，O-PS血清学为区分这两种弗朗西斯菌提供了一种实用方法。