海洋细菌假交替单胞菌属KMM 634酸性O-特异性多糖的结构
Structure of an acidic O-specific polysaccharide of the marine bacterium Pseudoalteromonas sp. KMM 634.
作者信息
Komandrova N A, Tomshich S V, Shevchenko L S, Perepelov A V, Senchenkova S N, Shashkov A S, Knirel Y A
机构信息
Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia.
出版信息
Biochemistry (Mosc). 2000 Sep;65(9):1060-7.
An acidic O-specific polysaccharide containing D-glucuronic acid (D-GlcA), 2,3-diacetamido-2,3-dideoxy-D-glucuronic acid (D-GlcNAc3NAcA), 2,3-diacetamido-2,3-dideoxy-D-mannuronoyl-L-alanine (D-ManNAc3NAcA6Ala), and 2-acetamido-2,4, 6-trideoxy-4-[(S)-3-hydroxybutyramido]-D-glucose (D-QuiNAc4NAcyl) was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Pseudoalteromonas sp. KMM 634 followed by gel-permeation chromatography. The polysaccharide was cleaved selectively with a new solvolytic agent, trifluoromethanesulfonic acid, to give a disaccharide and a trisaccharide with D-GlcNAc3NAcA at the reducing end. The borohydride-reduced oligosaccharides and the initial polysaccharide were studied by GLC-MS and 1H- and 13C-NMR spectroscopy, and the following structure of the linear tetrasaccharide repeating unit of the polysaccharide was established: -->3)-alpha-D-QuipNAc4Ac4NAcyl-(1-->4)-beta-D-ManpNAc3NAcA6Ala+ ++-(1-->4)-b eta-D-GlcpNAc3NAc3NAcA-(1-->4)-beta-D-GlcpA-(1-->.
通过对假交替单胞菌属细菌KMM 634的脂多糖进行温和酸降解，然后进行凝胶渗透色谱法，得到了一种酸性O-特异性多糖，其含有D-葡萄糖醛酸（D-GlcA）、2,3-二乙酰氨基-2,3-二脱氧-D-葡萄糖醛酸（D-GlcNAc3NAcA）、2,3-二乙酰氨基-2,3-二脱氧-D-甘露糖醛酰-L-丙氨酸（D-ManNAc3NAcA6Ala）和2-乙酰氨基-2,4,6-三脱氧-4-[(S)-3-羟基丁酰胺基]-D-葡萄糖（D-QuiNAc4NAcyl）。用一种新型溶剂解剂三氟甲磺酸对该多糖进行选择性裂解，得到一种二糖和一种还原端带有D-GlcNAc3NAcA的三糖。通过气相色谱-质谱联用仪（GLC-MS）以及1H-和13C-核磁共振光谱对硼氢化钠还原的寡糖和初始多糖进行了研究，并确定了该多糖线性四糖重复单元的以下结构：-->3)-α-D-奎诺糖胺4Ac4NAcyl-(1-->4)-β-D-甘露糖胺3NAcA6Ala+ ++-(1-->4)-β-D-葡萄糖胺3NAc3NAcA-(1-->4)-β-D-葡萄糖醛酸-(1-->。
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