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图莱里弗朗西斯菌15株O抗原的结构

Structure of the O-antigen of Francisella tularensis strain 15.

作者信息

Vinogradov E V, Shashkov A S, Knirel Y A, Kochetkov N K, Tochtamysheva N V, Averin S F, Goncharova O V, Khlebnikov V S

机构信息

N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the U.S.S.R., Moscow.

出版信息

Carbohydr Res. 1991 Jul 30;214(2):289-97. doi: 10.1016/0008-6215(91)80036-m.

DOI:10.1016/0008-6215(91)80036-m

PMID:1769021

Abstract

The O-specific polysaccharide, obtained by mild acid degradation of the lipopolysaccharide of Francisella tularensis strain 15, contained 2-acetamido-2,6-dideoxy-D-glucose (D-QuiNAc), 4,6-dideoxy-4-formamido-D-glucose (D-Qui4NFm), and 2-acetamido-2-deoxy-D-galacturonamide (D-GalNAcAN) in the ratios 1:1:2. Tri- and tetra-saccharide fragments were obtained on treatment of the polysaccharide with anhydrous hydrogen fluoride and partial hydrolysis with 0.1 M hydrochloric acid, respectively. On the basis of 1H- and 13C-n.m.r. spectroscopy of the polysaccharide and the saccharides, it was concluded that the O-antigen had the structure: ----4)-alpha-D-GalpNAcAN-(1----4)-alpha-D-GalpNAcAN-(1----3) -beta-D-QuipNAc-(1----2)-beta-D-Quip4NFm-(1----. This O-antigen is related in structure to those of Pseudomonas aeruginosa O6, immunotype 1, and IID 1008, and Shigella dysenteriae type 7.

摘要

通过对土拉弗朗西斯菌15株脂多糖进行温和酸降解获得的O-特异性多糖，含有2-乙酰氨基-2,6-二脱氧-D-葡萄糖（D-QuiNAc）、4,6-二脱氧-4-甲酰胺基-D-葡萄糖（D-Qui4NFm）和2-乙酰氨基-2-脱氧-D-半乳糖醛酰胺（D-GalNAcAN），其比例为1:1:2。分别用无水氟化氢处理该多糖和用0.1 M盐酸进行部分水解，得到了三糖和四糖片段。基于该多糖和糖类的1H-和13C-核磁共振光谱，得出O-抗原具有以下结构：----4)-α-D-GalpNAcAN-(1----4)-α-D-GalpNAcAN-(1----3)-β-D-QuipNAc-(1----2)-β-D-Quip4NFm-(1----。这种O-抗原在结构上与铜绿假单胞菌O6、免疫型1和IID 1008以及痢疾志贺菌7型的O-抗原相关。