Pfisterer J
Z Naturforsch C Biosci. 1978 May-Jun;33(5-6):359-62. doi: 10.1515/znc-1978-5-609.
Messenger ribonucleoprotein particles were isolated from the polysomes of logarithmically growing plant cell cultures, pulse-labeled with [3H] adenosine for 30 min. More than 80% of the labeled RNP was present in particles sedimenting between 80 S and 30 S on sucrose density gradients, but was not associated with ribosomal subunits. The size distribution differs from those reported for polysomal mRNP particles to date. After fixation with glutaraldehyde the labeled RNP particles had a buoyant density of 1.38 g/cm3 in CsCl gradients. Radioactively labeled RNA extracted from the RNP particles showed a heterodisperse size distribution and contained poly (A) stretches as determined by affinity chromatography and ribonuclease digestion experiments.
从对数生长期植物细胞培养物的多核糖体中分离出信使核糖核蛋白颗粒，用[3H]腺苷脉冲标记30分钟。超过80%的标记核糖核蛋白存在于蔗糖密度梯度上沉降于80S和30S之间的颗粒中，但不与核糖体亚基结合。其大小分布与迄今为止报道的多核糖体信使核糖核蛋白颗粒的不同。用戊二醛固定后，标记的核糖核蛋白颗粒在CsCl梯度中的浮力密度为1.38 g/cm3。从核糖核蛋白颗粒中提取的放射性标记RNA显示出异质的大小分布，并且通过亲和色谱和核糖核酸酶消化实验确定含有多聚（A）序列。
