通过寡聚脱氧胸苷酸 - 纤维素色谱法从粗糙脉孢菌多聚核糖体中分离出的信使核糖核蛋白复合物。
Messenger ribonucleoprotein complexes isolated by oligodeoxythymidylate-cellulose chromatography from Neurospora crassa polysomes.
作者信息
Mirkes P E
出版信息
J Bacteriol. 1977 Jul;131(1):240-6. doi: 10.1128/jb.131.1.240-246.1977.
Messenger ribonucleoprotein (mRNP) complexes were isolated from ethylenediaminetetraacetic acid-dissociated polysomes of Neurospora crassa. Approximately 15% of the [3H]uridine incorporated into polysomal ribonucleic acid (RNA) during a 15-min pulse was eluted from oligodeoxythymidylate-cellulose as an mRNP complex. The isolated mRNP complexes exhibited sedimentation coefficients ranging from 15S to greater than 60S. RNA isolated from these mRNP complexes sedimented in sucrose gradients between 4S and 40S, with broad peaks at 15S and 24S. The buoyant density of mRNP complexes eluted with 25% formamide was 1.42 to 1.44 g/cm3, whereas for mRNP complexes eluted with 50% formamide it was 1.48 to 1.50 g/cm3. Six polypeptides, with molecular weights of 14,000, 19,000, 24,000, 31,000, 44,000, and 66,000, were associated with mRNP complexes eluted with 25% formamide. The mRNP complexes eluted with 50% formamide had one associated polypeptide, of molecular weight 27,000.
从粗糙脉孢菌经乙二胺四乙酸解离的多核糖体中分离出信使核糖核蛋白（mRNP）复合物。在15分钟的脉冲标记期间，掺入多核糖体核糖核酸（RNA）中的[3H]尿苷约15%作为mRNP复合物从寡聚脱氧胸苷纤维素上洗脱下来。分离出的mRNP复合物的沉降系数范围为15S至大于60S。从这些mRNP复合物中分离出的RNA在蔗糖梯度中的沉降范围为4S至40S，在15S和24S处有宽峰。用25%甲酰胺洗脱的mRNP复合物的浮力密度为1.42至1.44 g/cm³，而用50%甲酰胺洗脱的mRNP复合物的浮力密度为1.48至1.50 g/cm³。六种分子量分别为14,000、19,000、24,000、31,000、44,000和66,000的多肽与用25%甲酰胺洗脱的mRNP复合物相关联。用50%甲酰胺洗脱的mRNP复合物有一种分子量为27,000的相关多肽。
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