Du Zhi-qian, Du Tian-xin, Wang Zhong-dong, Li Gen-lin
Henan Provencial Institute of Luoyang Orthopedics, Luoyang 471002, Henan, China.
Zhongguo Zhong Yao Za Zhi. 2003 Jan;28(1):32-4.
To select the optimum extraction process of Zhanjin Ruji.
To observe influence of extraction time upon the extraction rate of volatile oil, the orthogonal test was adopted to observe the extraction process by alcohol from the extraction rate and content of the total saponins in Radix Notoginseng.
The three kinds of herbs including Radix Angelicae Sinensis, Resina Olibani and Myrrha were extracted with water for 3 hours, 95% of volatile oil can be distilled. The three kinds of herbs including Radix Notoginseng, Herba Lycopodii and Radix Gentianae Macrophyllac were extracted by alcohol. Four factors such as alcohol concentration(A), extraction times(B), extraction time(C), and solvent amount(D), had not significant effect on the content of total saponins in Radix Notoginseng in herbal extraction, but factor A and B had significant effect on the extraction rate. The optimum extraction process was as follows extracted with 5 times the amount of the solvent volum 60% alcohol for 3 times and with each time for 1 hour. Three times experiments showed that the extraction rate was 26.5% and the content of the total saponins in Radix Notoginseng was 17.28% mg.g-1.
The above experimental results can provide experimental basis for deciding the extraction process of Zhanjin Ruji.
