Pan Gui-xiang, Zhang Bo-li, Gao Xiu-mei
Tianjin College of Traditional Chinese Medicine, Tianjin 300193, China.
Zhongguo Zhong Yao Za Zhi. 2003 Jun;28(6):562-5.
To investigate the absorption of the water soluble components of Salvia miltiorrhiza and establish methods to determine Danshensu in rat serum after ig compound Salvia recipe.
Used liquid-liquid extraction to deal with the serum, then determined the sample by high-performance liquid chromatography. The conditions for HPLC were as the following: the mobile phase, CH3OH-CH3CN-0.5% CH3COOH (2.5:3.5:94) with a flow rate of 1.0 mL.min-1; detection wavelength, 281 nm; internal standard, 4-hydroxy-benzoic acid.
Danshensu and an unknown compound whose reserved time was little shorter than protocatechuic aldehyde were found in the rat serum after ig compound Salvia recipe, while protocatechuic aldehyde weren't found in the serum.
Danshensu can be absorbed into blood after ig compound Salvia recipe.
研究丹参水溶性成分的吸收情况,并建立灌胃复方丹参方后大鼠血清中丹参素的测定方法。
采用液-液萃取法处理血清,然后用高效液相色谱法测定样品。高效液相色谱条件如下:流动相为甲醇-乙腈-0.5%醋酸(2.5:3.5:94),流速为1.0 mL·min-1;检测波长为281 nm;内标为4-羟基苯甲酸。
灌胃复方丹参方后大鼠血清中检测到丹参素和一个保留时间略短于原儿茶醛的未知化合物,而血清中未检测到原儿茶醛。
灌胃复方丹参方后丹参素可吸收入血。