Johansson Karl-Erik, Duhamel Gerald E, Bergsjö Bjarne, Engvall Eva Olsson, Persson Marianne, Pettersson Bertil, Fellström Claes
National Veterinary Institute, Department of Bacteriology, SE-75189 and Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden 2Department of Veterinary and Biochemical Sciences, University of Nebraska-Lincoln, USA 3Department of Bacteriology, National Veterinary Institute, Oslo, Norway 4Department of Disease Control and Biosecurity, National Veterinary Institute, Uppsala, Sweden 5Department of Biochemistry and Biotechnology, The Royal Institute of Technology, Stockholm, Sweden 6Department of Large Animal Clinical Sciences, Swedish University of Agricultural Sciences, Box 7018, SE-75007, Uppsala, Sweden.
J Med Microbiol. 2004 Apr;53(Pt 4):345-350. doi: 10.1099/jmm.0.05479-0.
It has been suggested that canine intestinal spirochaetes consist of Brachyspira pilosicoli and a group of strains that has been provisionally designated 'Brachyspira canis'. The purpose of the present study was to compare 22 spirochaete isolates that were obtained from intestinal specimens of dogs in Sweden (n = 12), Norway (n = 4), the United States (n = 3), Australia (n = 2) and Germany (n = 1) with type and reference strains, as well as field isolates, of Brachyspira species by five biochemical tests and determination of almost-complete 16S rDNA sequences. In an evolutionary tree derived from 16S rDNA sequences, the canine isolates grouped into three clusters. One cluster included the type strain of porcine B. pilosicoli, whereas a second larger cluster, which was monophyletic, contained a canine strain that was identified previously as 'B. canis'. The third cluster consisted of three canine isolates of Scandinavian origin, which grouped together with the type strain of the species Brachyspira alvinipulli (pathogenic to chicken). These three genotypes, which were identified on the basis of 16S rDNA sequences, corresponded to four phenotypic groups based on biochemical testing. Two biochemical tests, hippurate hydrolysis and alpha-galactosidase production, were sufficient for rapid identification of each canine cluster.
有人提出犬肠道螺旋体由柔毛短螺旋体和一组暂定为“犬短螺旋体”的菌株组成。本研究的目的是通过五项生化试验和测定几乎完整的16S rDNA序列，将从瑞典（n = 12）、挪威（n = 4）、美国（n = 3）、澳大利亚（n = 2）和德国（n = 1）的犬肠道标本中获得的22株螺旋体分离株与短螺旋体属的模式菌株、参考菌株以及野外分离株进行比较。在由16S rDNA序列推导的进化树中，犬分离株分为三个簇。一个簇包括猪柔毛短螺旋体的模式菌株，而第二个较大的单系簇包含一个先前被鉴定为“犬短螺旋体”的犬菌株。第三个簇由三个斯堪的纳维亚起源的犬分离株组成，它们与阿尔文短螺旋体（对鸡致病）的模式菌株聚在一起。基于16S rDNA序列鉴定的这三种基因型，根据生化测试对应于四个表型组。两项生化试验，即马尿酸盐水解和α-半乳糖苷酶产生，足以快速鉴定每个犬簇。
