用Gal4/UAS-GFP可视化的果蝇胆碱能神经元及突起。

Drosophila cholinergic neurons and processes visualized with Gal4/UAS-GFP.

作者信息

Salvaterra P M, Kitamoto T

机构信息

Division of Neuroscience, Beckman Research Institute of the City of Hope, 1450 E. Duarte Road, Duarte, CA 91010, USA.

出版信息

Brain Res Gene Expr Patterns. 2001 Aug;1(1):73-82. doi: 10.1016/s1567-133x(01)00011-4.

DOI:10.1016/s1567-133x(01)00011-4

PMID:15018821

Abstract

Using 7.4 kb of 5' flanking DNA from the Drosophila cholinergic gene locus to drive Gal4 expression we can visualize essentially all cholinergic neurons and neuropiles after genetic recombination with a UAS-GFP (S65T) reporter gene. In contrast to previous methods somata and neuropiles can be observed in the same samples. Fluorescence intensity is strong enough to allow observations in live animals at all developmental stages. Three-dimensional reconstructions made from confocal sections of whole-mount preparations reveal the extensive cholinergic connections among various regions of the nervous system.

摘要

利用来自果蝇胆碱能基因位点的7.4 kb 5'侧翼DNA来驱动Gal4表达，在与UAS-GFP（S65T）报告基因进行基因重组后，我们基本上可以可视化所有胆碱能神经元和神经纤维网。与以前的方法不同，可以在同一样本中观察到胞体和神经纤维网。荧光强度足够强，能够在所有发育阶段的活体动物中进行观察。从整装标本的共聚焦切片进行的三维重建揭示了神经系统各个区域之间广泛的胆碱能连接。