与骨和软组织肿瘤晚期基因组变化相关的有丝分裂不稳定性。

Mitotic instability associated with late genomic changes in bone and soft tissue tumours.

作者信息

Gisselsson David, Pålsson Eva, Yu Christina, Mertens Fredrik, Mandahl Nils

机构信息

Department of Clinical Genetics, University Hospital, Lund SE-221 85, Sweden.

出版信息

Cancer Lett. 2004 Mar 31;206(1):69-76. doi: 10.1016/j.canlet.2003.10.022.

DOI:10.1016/j.canlet.2003.10.022

PMID:15019162

Abstract

One source of genomic instability in tumours is abnormal mitotic segregation of chromosomes. Evaluation of chromosome segregation and cytogenetic aberrations in 28 bone and soft tissue neoplasms revealed few mitotic disturbances in benign lesions, whereas most of the malignant tumours, except for chondrosarcomas, showed anaphase bridges and/or multipolar mitoses. Only cases with chromosomal imbalances exhibited these defects and they were not present in any of the cases with sole primary changes, indicating that mitotic instability is established relatively late in mesenchymal tumour development. Most cases with multipolar mitoses exhibited abnormal centrosome configurations. However, induction of supernumerary centrioles in vitro failed to produce mitotic abnormalities in normal cells, indicating that additional disturbances of the cell division machinery are required for the generation of mitotic multipolarity.

摘要

肿瘤基因组不稳定的一个来源是染色体的异常有丝分裂分离。对28例骨和软组织肿瘤的染色体分离及细胞遗传学畸变进行评估后发现，良性病变中几乎没有有丝分裂紊乱，而除软骨肉瘤外的大多数恶性肿瘤均显示后期桥和/或多极有丝分裂。只有染色体失衡的病例出现这些缺陷，而单一原发性改变的病例均未出现，这表明有丝分裂不稳定性在间充质肿瘤发展过程中建立得相对较晚。大多数有多极有丝分裂的病例表现出异常的中心体构型。然而，体外诱导多余中心粒未能在正常细胞中产生有丝分裂异常，这表明产生有丝分裂多极性还需要细胞分裂机制的其他紊乱。