Pinheiro Carla Aparecida, Soares Iberê Cauduro, Penna Valter, Squire Jeremy, Reis Rui Manuel Vieira, da Silva Sandra Regina Morini, de Carvalho Isabela, Camparoto Marjori Leiva, da Silva Maicon Fernando Zanon, Longatto Filho Adhemar Longatto
Barretos Cancer Hospital, Pio XII Foundation, Barretos, São Paulo 14780-000, Brazil.
Department of Orthopedics, Faculty of Medicine, Federal University of Uberlândia, Uberlândia, Minas Gerais 38408-100, Brazil.
Oncol Lett. 2017 Mar;13(3):1835. doi: 10.3892/ol.2017.5633. Epub 2017 Jan 23.
The genetics background underlying the aggressiveness of chondrosarcoma (CS) is poorly understood. One possible cause of malignant transformation is chromosomal instability, which involves an error in mitotic segregation due to numerical and/or functional abnormalities of centrosomes. The present study aimed to evaluate centrosome amplification in cryopreserved samples of tumor tissue from patients with CS. An analysis was performed on 3 primary cultures of tumors from patients who underwent surgery between January 2012 and December 2012 at the Department of Orthopedics at the Barretos Cancer Hospital (Barretos, Brazil). Additionally, cryopreserved tumor specimens were analyzed from 10 patients. The data were assessed using immunocytochemistry and immunohistochemistry staining techniques with monoclonal antibody anti-γ-tubulin. A total of 4 samples of CS cultured cells were obtained from 3 patients. A recurrence of a histological grade III tumor was detected in a female patient with Ollier's syndrome. The other 2 cases were grade I and III. The incidence of centrosome amplification in the primary cultures ranged from 15-64% of the cells. Whereas control cultured fibroblasts showed baseline levels of 4% amplified cells. For the cryopreserved specimens, two independent observers analyzed each sample and counted the cells stained with γ-tubulin, verifying the percentage of affected cells to be a mean of 14%, with the number of clusters ranging between 0-6 per slide. In conclusion, centrosome amplification was found to be a consistent biological feature of CS and may underlie chromosomal instability in this tumor.
软骨肉瘤（CS）侵袭性的遗传学背景尚不清楚。恶性转化的一个可能原因是染色体不稳定，这涉及由于中心体的数量和/或功能异常导致的有丝分裂分离错误。本研究旨在评估CS患者肿瘤组织冷冻保存样本中的中心体扩增情况。对2012年1月至2012年12月在巴西巴雷图癌症医院骨科接受手术的患者的3个肿瘤原代培养物进行了分析。此外，还分析了10例患者的冷冻保存肿瘤标本。使用抗γ-微管蛋白单克隆抗体通过免疫细胞化学和免疫组织化学染色技术评估数据。从3例患者中获得了总共4个CS培养细胞样本。在一名患有Ollier综合征的女性患者中检测到组织学III级肿瘤复发。另外2例为I级和III级。原代培养物中中心体扩增的发生率在细胞的15%-64%之间。而对照培养的成纤维细胞显示扩增细胞的基线水平为4%。对于冷冻保存的标本，两名独立的观察者分析每个样本并对用γ-微管蛋白染色的细胞进行计数，验证受影响细胞的百分比平均为14%，每张载玻片上的簇数在0-6之间。总之，发现中心体扩增是CS的一个一致生物学特征，可能是该肿瘤染色体不稳定的基础。
