Ruley Kristin M, Ansede John H, Pritchett Christopher L, Talaat Adel M, Reimschuessel Renate, Trucksis Michele
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
FEMS Microbiol Lett. 2004 Mar 12;232(1):75-81. doi: 10.1016/S0378-1097(04)00017-5.
Mycobacterium marinum, a causative agent of fish tuberculosis, is one of the most closely related Mycobacterium species (outside the M. tuberculosis complex) to M. tuberculosis, the etiologic agent of human tuberculosis. Signature-tagged mutagenesis was used to identify genes of M. marinum required for in vivo survival in a goldfish model of mycobacterial pathogenesis. Screening the first 1008 M. marinum mutants led to the identification of 40 putative virulence mutants. DNA sequence analysis of these 40 mutants identified transposon insertions in 35 unique loci. Twenty-eight out of 33 (85%) loci encoding putative virulence genes have homologous genes in M. tuberculosis.
海分枝杆菌是鱼类结核病的病原体，是（在结核分枝杆菌复合群之外）与人类结核病的病原体结核分枝杆菌关系最密切的分枝杆菌物种之一。采用基因标签诱变技术，在金鱼分枝杆菌致病模型中鉴定出海分枝杆菌在体内存活所需的基因。对最初的1008个海分枝杆菌突变体进行筛选，鉴定出40个假定的毒力突变体。对这40个突变体的DNA序列分析确定了转座子插入35个独特位点。在编码假定毒力基因的33个位点中，有28个（85%）在结核分枝杆菌中有同源基因。
Zotero 插件