Ju Y Y, Womersley H, Pritchard J, Gray I, Hughes R A C, Gregson N A
Department of Clinical Neurosciences, Guy's, King's, and St. Thomas' School of Medicine, Guy's Hospital, Hodgkin Building, St. Thomas' Street, London SE1 1UL, UK.
J Neuroimmunol. 2004 Apr;149(1-2):160-6. doi: 10.1016/j.jneuroim.2003.12.011.
Recent reports have contained conflicting results on the relationship between antecedent Haemophilus influenzae infection and Guillain-Barré syndrome (GBS). To investigate the prevalence of H. influenzae infection in GBS patients in a British population, we carried out a retrospective study with 62 consecutive GBS patients and 63 normal controls of similar age and sex. Whole bacteria of both encapsulated and nonencapsulated strains of H. influenzae were employed as antigens in an enzyme-linked immunosorbent assay (ELISA) for anti-H. influenzae IgG, IgM and IgA antibodies. Elevated antibodies of two or three classes were found in one GBS patient and none in the normal controls. Six GBS patients had IgG antibodies against nonencapsulated H. influenzae compared with only one in the normal control group (p=0.06). Western blot for IgG antibody showed that all the sera with IgG antibodies recognized the lipopolysaccharide (LPS) of both strains of H. influenzae. Antiganglioside GM1 antibody was not associated with anti-H. influenzae antibody in our study. Absorption with encapsulated or nonencapsulated H. influenzae, Campylobacter jejuni and Escherichia coli before testing on Western blot showed that only nonencapsulated H. influenzae absorbed the anti-LPS antibodies. In conclusion, there is a possible but rare association of GBS with nonencapsulated H. influenzae in the UK.
近期报告中关于先前感染流感嗜血杆菌与吉兰-巴雷综合征（GBS）之间的关系存在相互矛盾的结果。为了调查英国人群中GBS患者流感嗜血杆菌感染的患病率，我们对62例连续的GBS患者和63例年龄和性别相似的正常对照进行了一项回顾性研究。流感嗜血杆菌的包膜菌株和非包膜菌株的全菌均用作酶联免疫吸附测定（ELISA）中抗流感嗜血杆菌IgG、IgM和IgA抗体的抗原。1例GBS患者发现两类或三类抗体升高，正常对照中未发现此类情况。6例GBS患者有针对非包膜流感嗜血杆菌的IgG抗体，而正常对照组只有1例（p = 0.06）。IgG抗体的蛋白质印迹分析表明，所有含IgG抗体的血清均识别两种流感嗜血杆菌菌株的脂多糖（LPS）。在我们的研究中，抗神经节苷脂GM1抗体与抗流感嗜血杆菌抗体无关。在蛋白质印迹检测前用包膜或非包膜流感嗜血杆菌、空肠弯曲菌和大肠杆菌进行吸收试验表明，只有非包膜流感嗜血杆菌能吸收抗LPS抗体。总之，在英国GBS与非包膜流感嗜血杆菌之间可能存在一种罕见的关联。
