Byers John A
USDA-ARS Western Cotton Research Laboratory, 4135 E. Broadway Road, Phoenix, AZ 85040, USA.
J Neurosci Methods. 2004 May 30;135(1-2):89-93. doi: 10.1016/j.jneumeth.2003.12.004.
Heating of chromatographic columns, transfer lines, and other devices is often required in neuroscience research. For example, volatile compounds passing through a capillary column of a gas chromatograph (GC) can be split, with half exiting the instrument through a heated transfer line to an insect antenna or olfactory sensillum for electroantennographic detector (GC-EAD) recordings. The heated transfer line is used to prevent condensation of various chemicals in the capillary that would otherwise occur at room temperature. Construction of such a transfer line heater is described using (80/20%) nickel-chromium heating wire wrapped in a helical coil and powered by a 120/220 V ac rheostat. Algorithms were developed in a computer program to estimate the voltage at which a rheostat should be set to obtain the desired heater temperature for a specific coil. The coil attributes (radius, width, number of loops, or length of each loop) are input by the user, as well as AWG size of heating wire and desired heater temperature. The program calculates total length of wire in the helix, resistance of the wire, amperage used, and the voltage to set the rheostat. A discussion of semiochemical isolation methods using the GC-EAD and bioassays is presented.
在神经科学研究中，通常需要对色谱柱、传输线和其他设备进行加热。例如，通过气相色谱仪（GC）毛细管柱的挥发性化合物可以被分流，其中一半通过加热的传输线离开仪器，连接到昆虫触角或嗅觉感受器，用于进行触角电位检测（GC-EAD）记录。加热的传输线用于防止毛细管中各种化学物质在室温下发生冷凝。本文描述了一种传输线加热器的构造，它使用（80/20%）镍铬加热丝缠绕成螺旋线圈，并由120/220 V交流变阻器供电。在计算机程序中开发了算法，以估计为特定线圈获得所需加热器温度时应设置变阻器的电压。用户输入线圈属性（半径、宽度、匝数或每个线圈的长度），以及加热丝的美国线规尺寸和所需的加热器温度。该程序计算螺旋中导线的总长度、导线的电阻、使用的电流以及设置变阻器的电压。本文还讨论了使用GC-EAD和生物测定法的信息素分离方法。
