Park Eui Kyun, Lee Young Eun, Choi Je-Yong, Oh Sun-Ho, Shin Hong-In, Kim Kyo-Han, Kim Shin-Yoon, Kim Sukyoung
Skeletal Diseases Research Center, Kyungpook National University Hospital, Daegu 700-412, South Korea.
Biomaterials. 2004 Aug;25(17):3403-11. doi: 10.1016/j.biomaterials.2003.10.031.
In the present study, the in vitro biocompatibility of calcium metaphosphate (CMP) with human bone marrow stromal cells (HBMSCs) and its effect on osteoblastic differentiation have been investigated. Powder and disk forms of CMP do not exert a cytotoxic effect on the HBMSCs undergoing osteoblastic differentiation. In addition, the HBMSCs adhere to the surface of the CMP disk as successfully as to the culture plate or hydroxyapatite (HA) disk. The HBMSCs adhered to either the HA or CMP disk display an undistinguishable actin arrangement and cellular phenotypes, indicating that the CMP does not disrupt normal cellular responses. An analysis of the differentiation of the HBMSCs cultured on culture plate, the HA and the CMP disk shows that three matrices are capable of supporting osteoblastic differentiation of the HBMSCs as accessed by alkaline phosphatase (ALP) staining. Further molecular analysis of osteoblastic differentiation of HBMSCs reveals that the CMP disk has a better ability than the HA disk to induce an expression of osteoblast-related genes, including ALP, osteoprotegerin (OPG), a decoy receptor for RANK ligand, and osteopontin (OPN), a non-collagenous bone matrix protein. The results demonstrate that, in addition to favorable biocompatibility, the CMP can stimulate osteoblastic differentiation of the HBMSCs in vitro.
在本研究中，已对偏磷酸钙（CMP）与人骨髓基质细胞（HBMSCs）的体外生物相容性及其对成骨细胞分化的影响进行了研究。CMP的粉末和圆盘形式对正在进行成骨细胞分化的HBMSCs没有细胞毒性作用。此外，HBMSCs在CMP圆盘表面的黏附情况与在培养板或羟基磷灰石（HA）圆盘上的黏附情况一样成功。黏附在HA或CMP圆盘上的HBMSCs表现出难以区分的肌动蛋白排列和细胞表型，这表明CMP不会破坏正常的细胞反应。对在培养板、HA和CMP圆盘上培养的HBMSCs的分化情况进行分析表明，通过碱性磷酸酶（ALP）染色可知，这三种基质都能够支持HBMSCs的成骨细胞分化。对HBMSCs成骨细胞分化的进一步分子分析表明，CMP圆盘比HA圆盘具有更好的诱导成骨细胞相关基因表达的能力，这些基因包括ALP、骨保护素（OPG，一种RANK配体的诱饵受体）和骨桥蛋白（OPN，一种非胶原骨基质蛋白）。结果表明，除了具有良好的生物相容性外，CMP还能在体外刺激HBMSCs的成骨细胞分化。
