Sun Hongli, Wu Chengtie, Dai Kerong, Chang Jiang, Tang Tingting
Orthopaedic Cellular & Molecular Biology Laboratory, Laboratory of Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences & Shanghai JiaoTong University School of Medicine, People's Republic of China.
Biomaterials. 2006 Nov;27(33):5651-7. doi: 10.1016/j.biomaterials.2006.07.027. Epub 2006 Aug 14.
In the present study, the effects of a calcium magnesium silicate bioactive ceramic (akermanite) on proliferation and osteoblastic differentiation of human bone marrow stromal cells (hBMSC) have been investigated and compared with the classical ceramic (beta-tricalcium phosphate, beta-TCP). Akermanite and beta-TCP disks were seeded with hBMSC and kept in growth medium or osteogenic medium for 10 days. Proliferation and osteoblastic differentiation were evaluated on day 1, 4, 7 and 10. The data from the Alamar Blue assay and lactic acid production assay showed that hBMSC proliferated more significantly on akermanite than on beta-TCP. The analysis of osteoblast-related genes, including alkaline phosphatase (ALP), osteopontin (OPN), bone sialoprotein (BSP) and osteocalcin (OC), indicated that akermanite ceramics enhanced the expression of osteoblast-related genes, but type I collagen (COL I) showed no noticeable difference among akermanite and beta-TCP ceramics. Furthermore, this stimulatory effect was observed not only in osteogenic medium, but also in normal growth medium without osteogenic reagents such as l-ascorbic acid, glycerophosphate and dexamethasone. This result suggests that akermanite can promote osteoblastic differentiation of hBMSC in vitro even without osteogenic reagents, and may be used as a bioactive material for bone regeneration and tissue engineering applications.
在本研究中，已对硅酸钙镁生物活性陶瓷（钙黄长石）对人骨髓基质细胞（hBMSC）增殖和成骨分化的影响进行了研究，并与传统陶瓷（β-磷酸三钙，β-TCP）进行了比较。将hBMSC接种到钙黄长石和β-TCP盘上，并置于生长培养基或成骨培养基中培养10天。在第1、4、7和10天评估增殖和成骨分化情况。阿拉玛蓝测定法和乳酸生成测定法的数据表明，hBMSC在钙黄长石上的增殖比在β-TCP上更显著。对成骨细胞相关基因的分析，包括碱性磷酸酶（ALP）、骨桥蛋白（OPN）、骨唾液蛋白（BSP）和骨钙素（OC），表明钙黄长石陶瓷增强了成骨细胞相关基因的表达，但I型胶原蛋白（COL I）在钙黄长石和β-TCP陶瓷之间没有明显差异。此外，不仅在成骨培养基中观察到这种刺激作用，在不含l-抗坏血酸、甘油磷酸酯和地塞米松等成骨试剂的正常生长培养基中也观察到了这种作用。这一结果表明，即使没有成骨试剂，钙黄长石也能在体外促进hBMSC的成骨分化，并可能用作骨再生和组织工程应用的生物活性材料。
