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Retrofitting BACs and PACs with LoxP transposons to generate nested deletions.

作者信息

Chatterjee Pradeep K

机构信息

Julius L. Chambers Biomedical/Biotechnology Research Institute, North Carolina Central University, Durham, USA.

出版信息

Methods Mol Biol. 2004;255:231-41. doi: 10.1385/1-59259-752-1:231.

DOI:10.1385/1-59259-752-1:231
PMID:15020829
Abstract
摘要

相似文献

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Retrofitting BACs and PACs with LoxP transposons to generate nested deletions.用LoxP转座子改造细菌人工染色体(BACs)和P1人工染色体(PACs)以产生嵌套缺失。
Methods Mol Biol. 2004;255:231-41. doi: 10.1385/1-59259-752-1:231.
2
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引用本文的文献

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Identifying Distal -acting Gene-Regulatory Sequences by Expressing BACs Functionalized with loxP-Tn10 Transposons in Zebrafish.通过在斑马鱼中表达用loxP-Tn10转座子功能化的BAC来鉴定远端作用的基因调控序列。
RSC Adv. 2013 Jun 21;3(23):8604-8617. doi: 10.1039/C3RA40332G.
2
Using BAC transgenesis in zebrafish to identify regulatory sequences of the amyloid precursor protein gene in humans.利用斑马鱼 BAC 转基因技术鉴定人类淀粉样前体蛋白基因的调控序列。
BMC Genomics. 2012 Sep 4;13:451. doi: 10.1186/1471-2164-13-451.
3
Generating libraries of iTol2-end insertions at BAC ends using loxP and lox511 Tn10 transposons.
利用 loxP 和 lox511 Tn10 转座子在 BAC 末端生成 iTol2 末端插入文库。
BMC Genomics. 2011 Jul 7;12:351. doi: 10.1186/1471-2164-12-351.
4
Replacing the wild type loxP site in BACs from the public domain with lox66 using a lox66 transposon.使用lox66转座子将公共领域BAC中的野生型loxP位点替换为lox66。
BMC Res Notes. 2010 Feb 19;3:38. doi: 10.1186/1756-0500-3-38.
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Context dependent function of APPb enhancer identified using enhancer trap-containing BACs as transgenes in zebrafish.使用含增强子捕获的细菌人工染色体作为转基因在斑马鱼中鉴定APPb增强子的上下文依赖性功能。
Nucleic Acids Res. 2008 Nov;36(19):6237-48. doi: 10.1093/nar/gkn628. Epub 2008 Oct 1.
6
Minimal cross-recombination between wild-type and loxP511 sites in vivo facilitates truncating both ends of large DNA inserts in pBACe3.6 and related vectors.野生型与loxP511位点在体内的最小交叉重组有助于截短pBACe3.6及相关载体中大片段DNA插入序列的两端。
Nucleic Acids Res. 2005 Aug 1;33(13):e118. doi: 10.1093/nar/gni119.
7
Mutually exclusive recombination of wild-type and mutant loxP sites in vivo facilitates transposon-mediated deletions from both ends of genomic DNA in PACs.野生型和突变型loxP位点在体内的互斥重组促进了转座子介导的从P1人工染色体(PACs)中基因组DNA两端的缺失。
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