Hitt J C, Feigal R J
University of Minnesota School of Dentistry, Minneapolis.
Pediatr Dent. 1992 Jan-Feb;14(1):41-6.
Moisture contamination of etched enamel during application of sealant is the most frequently cited reason for sealant failure. In preliminary studies, a dentin bonding agent (Scotchbond Dual Cure, 3M, St. Paul, MN) has been shown to bond adequately to etched enamel after salivary contamination. This study investigated bond strength in vitro, when a bonding agent was used beneath sealants under varied conditions of contamination. Five hundred bovine incisor crowns were separated randomly into eight groups. The enamel samples, etched for 60 sec with a 37% phosphoric acid gel, were contaminated with: 1) fresh whole saliva, air dried, 2) fresh whole saliva, left wet, or 3) moisture from a humidity chamber. All contamination conditions were tested for sealant bond strength with and without the bonding agent as an intermediate layer under the sealant. As controls, both sealant and bonding agent under sealant also were applied to clean etched enamel. Bond strength was measured using a universal testing machine. Data were analyzed using a two-way ANOVA. Under conditions of humidity or intact saliva, sealant alone showed significant reduction in bond strength (P less than 0.001). Bonding agent under sealant on wet contamination yielded bond strengths equivalent to the bond strength obtained when sealant was bonded directly to clean, etched enamel. Bonding agent used without contamination yielded bond strengths significantly greater than the bond strength obtained when using sealant alone without contamination (P less than 0.001). When the saliva was air dried onto the surface, there was no significant difference in bond strengths whether or not a bonding agent was used under the sealant.
在应用窝沟封闭剂时，蚀刻釉质受到水分污染是窝沟封闭剂失败最常被提及的原因。在初步研究中，一种牙本质粘结剂（Scotchbond双固化粘结剂，3M公司，明尼苏达州圣保罗）已被证明在受到唾液污染后能与蚀刻釉质充分粘结。本研究调查了在不同污染条件下，当粘结剂用于窝沟封闭剂下方时的体外粘结强度。500颗牛切牙冠随机分为八组。用37%磷酸凝胶蚀刻60秒后的釉质样本受到以下污染：1）新鲜全唾液，空气干燥；2）新鲜全唾液，保持湿润；或3）湿度箱中的水分。在有和没有粘结剂作为窝沟封闭剂下的中间层的情况下，对所有污染条件下的窝沟封闭剂粘结强度进行了测试。作为对照，窝沟封闭剂和窝沟封闭剂下的粘结剂也应用于清洁的蚀刻釉质。使用万能试验机测量粘结强度。数据采用双向方差分析进行分析。在湿度或完整唾液条件下，单独使用窝沟封闭剂时粘结强度显著降低（P小于0.001）。在潮湿污染情况下，窝沟封闭剂下的粘结剂产生的粘结强度与窝沟封闭剂直接粘结到清洁的蚀刻釉质时获得的粘结强度相当。无污染使用粘结剂时产生的粘结强度显著大于无污染单独使用窝沟封闭剂时获得的粘结强度（P小于0.001）。当唾液在表面空气干燥时，无论窝沟封闭剂下是否使用粘结剂，粘结强度均无显著差异。
