生物反应调节剂刺激后肝巨噬细胞（库普弗细胞）前列腺素E2生成的增强

Enhancement of prostaglandin E2 production by liver macrophages (Kupffer cells) after stimulation with biological response modifiers.

作者信息

Kawada N, Mizoguchi Y, Kobayashi K, Monna T, Liu P, Morisawa S

机构信息

Third Department of Internal Medicine, Osaka City University Medical School, Japan.

出版信息

Prostaglandins Leukot Essent Fatty Acids. 1992 Jun;46(2):105-10. doi: 10.1016/0952-3278(92)90216-6.

DOI:10.1016/0952-3278(92)90216-6

PMID:1502248

Abstract

PGE2 production by liver macrophages (Kupffer cells) activated by biological response modifiers was examined. Kupffer cells obtained from a normal rat liver possessed cyclooxygenase activity and produced TXB2, PGD2, and PGE2 from (1-14C)arachidonic acid. The major product was PGD2. When Kupffer cells were incubated in the presence of lipo-polysaccharide (LPS), OK-432, or heat-killed Propionibacterium acnes for 24 h, the amount of arachidonate cyclooxygenase products increased and the major product changed from PGD2 to PGE2. When liver macrophages including Kupffer cells were prepared from rats after an injection of LPS, OK-432, or heat-killed P. acnes, it was noticed that the number of cells obtained and PGE2 production increased compared with those of normal rat. These results suggested that PGE2 production by rat liver was induced when they were treated with biological response modifiers.

摘要

研究了生物反应调节剂激活的肝巨噬细胞（枯否细胞）产生前列腺素E2（PGE2）的情况。从正常大鼠肝脏获得的枯否细胞具有环氧化酶活性，能从（1-14C）花生四烯酸生成血栓素B2（TXB2）、前列腺素D2（PGD2）和PGE2。主要产物是PGD2。当枯否细胞在脂多糖（LPS）、OK-432或热灭活痤疮丙酸杆菌存在的情况下孵育24小时，花生四烯酸环氧化酶产物的量增加，主要产物从PGD2变为PGE2。当从注射LPS、OK-432或热灭活痤疮丙酸杆菌后的大鼠制备包括枯否细胞在内的肝巨噬细胞时，发现获得的细胞数量和PGE2产量与正常大鼠相比有所增加。这些结果表明，大鼠肝脏在用生物反应调节剂处理时会诱导产生PGE2。