Stehfest Katja, Boese Matthias, Kerns Gerhard, Piry Alexander, Wilhelm Christian
Institute of Botany, University of Leipzig, Johannisallee 21-23, D-04103 Leipzig, Germany.
J Plant Physiol. 2004 Feb;161(2):151-6. doi: 10.1078/0176-1617-01099.
A new procedure has been developed for the in situ FT-IR determination of rosmarinic acid (RA) in suspension cultures of Lavandula officinalis. The method involves sample preparation on ZnSe crystals or microplates from silicon, and measuring absorbance spectra between 4000 and 700 cm(-1). First derivative spectra were analysed after normalisation using partial least square (PLS) algorithm. The correlation between spectral analysis and HPLC measurements of cell extracts shows that the FT-IR procedure is suitable for qualitative and quantitative analyses of RA in cell suspension cultures.
已开发出一种新方法，用于原位傅里叶变换红外光谱法测定薰衣草悬浮培养物中的迷迭香酸（RA）。该方法包括在ZnSe晶体或硅制微孔板上制备样品，并测量4000至700 cm(-1)之间的吸收光谱。使用偏最小二乘法（PLS）算法进行归一化后，对一阶导数光谱进行分析。细胞提取物的光谱分析与高效液相色谱测量之间的相关性表明，傅里叶变换红外光谱法适用于细胞悬浮培养物中RA的定性和定量分析。
