Department of Science, Agricultural University of Athens, Athens, Greece.
J Agric Food Chem. 2013 Apr 3;61(13):3235-41. doi: 10.1021/jf305520m. Epub 2013 Mar 22.
For the determination of rosmarinic acid (RA) directly in pulverized plant material, a method is developed using diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) without any physicochemical pretreatment of samples. The RA content of 11 samples of eight different Lamiaceae herbs, as determined by high performance liquid chromatography (HPLC), varied between 86 ± 1 mg/g (in lemon balm) and 12.0 ± 0.8 mg/g (in hyssop) of dried plant material. The 11 samples and 50 other additional samples, which were prepared by mixing initial samples with KBr, were measured using DRIFTS. The second derivative of the spectral region 1344-806 cm(-1) was used and the corresponding data were analyzed using partial least squares (PLS) regression. The correlation between infrared spectral analysis and HPLC measurements shows that the DRIFTS method is sufficiently accurate, simple, and rapid. The RA content of the 11 Lamiaceae samples determined by DRIFTS ranged from 81 ± 4 mg/g (in lemon balm) to 12 ± 3 mg/g (in hyssop) of dried plant material.
为了直接在粉碎的植物材料中测定迷迭香酸（RA），开发了一种使用漫反射红外傅里叶变换光谱（DRIFTS）的方法，而无需对样品进行任何物理化学预处理。通过高效液相色谱（HPLC）测定的 11 种 8 种不同唇形科草药的 RA 含量，从干植物材料的 86 ± 1 mg/g（在柠檬香脂中）到 12.0 ± 0.8 mg/g（在牛膝草中）不等。使用 DRIFTS 测量了 11 个样品和 50 个其他额外的用 KBr 混合初始样品制备的样品。使用光谱区域 1344-806 cm(-1) 的二阶导数，并使用偏最小二乘（PLS）回归分析相应的数据。红外光谱分析与 HPLC 测量之间的相关性表明，DRIFTS 方法足够准确、简单和快速。通过 DRIFTS 测定的 11 种唇形科样品的 RA 含量范围为干植物材料的 81 ± 4 mg/g（在柠檬香脂中）至 12 ± 3 mg/g（在牛膝草中）。
