Meucci V, Gasperini A, Soldani G, Guidi G, Giorgi M
Pharmacology and Toxicology Section, Veterinary Clinics Department, V.le delle Piagge 2, 56124, Pisa, Italy.
J Chromatogr Sci. 2004 Feb;42(2):107-11. doi: 10.1093/chromsci/42.2.107.
A high-performance liquid chromatography method to determine iohexol (IOX) and p-aminohippuric acid (PAH) in the plasma of dogs is evaluated according to recovery, reproducibility, and linearity utilizing a gradient pump. The mobile phase consists of 50mM sodium dihydrogen phosphate with 0.5mM tetrabutylammonium chloride, the pH is adjusted to 4.1, methanol is added to the final ratio of 90:10 (v/v), the flow rate is set at 1 mL/min, and separation is achieved with an ODS2 Luna column. The UV detector is set at 254 nm. IOX and PAH are used for evaluation of the effective renal plasma flow (ERPF) and glomerular filtration rate (GFR). The present method tested in three dogs demonstrates the accuracy in the evaluation of ERPF and GFR. Because of its precision and simplicity and low cost, it can be considered a good tool for ERPF and GFR in small animal practice.
采用梯度泵，根据回收率、重现性和线性，对一种用于测定犬血浆中碘海醇（IOX）和对氨基马尿酸（PAH）的高效液相色谱法进行了评估。流动相由含有0.5mM四丁基氯化铵的50mM磷酸二氢钠组成，将pH值调至4.1，加入甲醇至终体积比为90:10（v/v），流速设定为1 mL/min，使用ODS2 Luna柱进行分离。紫外检测器设定在254 nm。IOX和PAH用于评估有效肾血浆流量（ERPF）和肾小球滤过率（GFR）。在三只犬身上测试的本方法证明了在评估ERPF和GFR方面的准确性。由于其精确性、简便性和低成本，它可被视为小动物临床中评估ERPF和GFR的良好工具。
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