阿拉伯胶对口服给予大鼠的等渗溶液的吸收抑制作用：对锌及其他溶质的影响。

Proabsorptive effect of gum arabic in isotonic solutions orally administered to rats: effect on zinc and other solutes.

作者信息

Ibrahim Mahmoud A, Kohn Nina, Wapnir Raul A

机构信息

Division of Perinatal/Neonatal Medicine, Schneider Children's Hospital at North Shore, North Shore-Long Island Jewish Health System, 300 Community Drive, Manhasset, NY 11030, USA.

出版信息

J Nutr Biochem. 2004 Mar;15(3):185-9. doi: 10.1016/j.jnutbio.2003.11.003.

DOI:10.1016/j.jnutbio.2003.11.003

PMID:15023401

Abstract

We have previously shown that the addition of gum arabic (GA) to oral rehydration solution (ORS) enhances water and electrolyte absorption during jejunal perfusion in rats under anesthesia. This study investigates whether GA by oral administration could be equally effective in rats. Isotonic solutions containing 25 g/L GA (AG), or without GA (A0) were administered via oral tube to lightly anesthetized adult female rats. Similar experiments were conducted with hypertonic solutions containing no GA (B0), or either 10 (B10) or 50 g/L GA (B50). Blood concentrations of sodium, glucose, glutamate, zinc, and tritiated water were determined at 0, 15, 30, 60, 90, 120, and 180 minutes, and results between treatments were compared. Administration of the isotonic, GA-containing solution (AG) resulted in a higher blood zinc level than with the isotonic GA-free solution (A0) from 15 minutes throughout 180 minutes. Blood zinc at 15 minutes (means +/- SEM) was as follows: for A0: 69.3 +/- 2.0, for AG: 83.4 +/- 3.5 nmol/L, P=0.002. At 180 minutes, A0: 52.6 +/- 1.8; AG: 68.1 +/- 4.6 nmol/L, P=0.004. The corresponding areas under the curve (AUC) were as follows: for A0: 10,737 +/- 214; for AG: 13,919 +/- 765 nmol x min/L, P<0.001). Glucose, glutamate, sodium, and tritiated water body distribution presented no differences in blood concentrations. For sodium and tritiated water body distribution, there was a significant time effect (P<0.0001). In hypertonic solutions, blood zinc levels declined over time, possibly due to their osmotic, counter-absorptive action, thus obscuring possible opposite effects of GA. GA appears to be an effective enhancer of zinc absorption when orally administered in isotonic solutions to laboratory animals. This proabsorptive capacity could be attributed to some of the physicochemical and biochemical properties of GA and suggest possible applications of GA in liquid formulas and solid food preparations.

摘要

我们之前已经表明，在麻醉大鼠的空肠灌注过程中，向口服补液溶液（ORS）中添加阿拉伯胶（GA）可增强水和电解质的吸收。本研究调查了口服GA对大鼠是否同样有效。通过胃管向轻度麻醉的成年雌性大鼠给予含有25 g/L GA的等渗溶液（AG）或不含GA的等渗溶液（A0）。对不含GA的高渗溶液（B0）、含有10 g/L GA的高渗溶液（B10）或含有50 g/L GA的高渗溶液（B50）进行了类似实验。在0、15、30、60、90、120和180分钟时测定血液中钠、葡萄糖、谷氨酸、锌和氚化水的浓度，并比较各处理之间的结果。从15分钟到180分钟，给予含GA的等渗溶液（AG）导致血液锌水平高于不含GA的等渗溶液（A0）。15分钟时的血液锌水平（平均值±标准误）如下：A0组为69.3±2.0，AG组为83.4±3.5 nmol/L，P = 0.002。在180分钟时，A0组为52.6±1.8；AG组为68.1±4.6 nmol/L，P = 0.004。相应的曲线下面积（AUC）如下：A0组为10,737±214；AG组为13,919±765 nmol·min/L，P<0.001）。葡萄糖、谷氨酸、钠和氚化水的体内分布在血液浓度上没有差异。对于钠和氚化水的体内分布，存在显著的时间效应（P<0.0001）。在高渗溶液中，血液锌水平随时间下降，可能是由于其渗透、反吸收作用，从而掩盖了GA可能产生的相反作用。当以等渗溶液口服给予实验动物时，GA似乎是锌吸收的有效增强剂。这种促进吸收的能力可能归因于GA的一些物理化学和生化特性，并提示GA在液体配方和固体食品制剂中的可能应用。