Kostanjšek Rok, Štrus Jasna, Drobne Damjana, Avguštin Gorazd
University of Ljubljana, Biotechnical Faculty, Biological Department, Večna pot 111, 1111 Ljubljana, Slovenia.
University of Ljubljana, Biotechnical Faculty, Zootechnical Department, Groblje 3, 1230 Domžale, Slovenia.
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):543-549. doi: 10.1099/ijs.0.02802-0.
Intracellular bacteria were observed in the hepatopancreas of the terrestrial isopod Porcellio scaber. Comparative 16S rRNA gene sequence analysis and electron microscopic observations were used to determine the taxonomic position of these intracellular bacteria. Phylogenetic analysis and a complex developmental cycle affiliate these bacteria to the order Chlamydiales, within which they form a distinctive lineage, close to the family Simkaniaceae. They share <92 % 16S rRNA gene sequence similarity with their closest relative and <88 % similarity with other members of the order Chlamydiales. A specific signature oligonucleotide sequence was identified and used as a probe, enabling the identification of intracellular bacteria in infected hepatopancreatic tissue. According to the distinctive morphology of their elementary bodies, which are rod-shaped rather than spherical and contain translucent oblong structures, their genomic properties and their crustacean host, the name 'Candidatus Rhabdochlamydia porcellionis' is proposed for intracellular bacteria in the hepatopancreas of P. scaber.
在陆生等足动物鼠妇（Porcellio scaber）的肝胰腺中观察到了细胞内细菌。利用16S rRNA基因序列比较分析和电子显微镜观察来确定这些细胞内细菌的分类地位。系统发育分析和复杂的发育周期表明这些细菌属于衣原体目，在该目中它们形成了一个独特的谱系，与西姆卡尼亚科（Simkaniaceae）亲缘关系较近。它们与最亲近的亲属的16S rRNA基因序列相似度小于92%，与衣原体目的其他成员的相似度小于88%。鉴定出了一个特异性的标志性寡核苷酸序列并将其用作探针，从而能够鉴定受感染肝胰腺组织中的细胞内细菌。根据其原体独特的形态（呈杆状而非球状，且含有半透明的长方形结构）、基因组特性以及甲壳类宿主，提议将鼠妇肝胰腺中的细胞内细菌命名为“类杆状衣原体（Candidatus Rhabdochlamydia porcellionis）”。
