Nevzglyadova Olga V, Artyomov Alexey V, Mikhailova Ekaterina V, Soidla Tonu R
Institute of Cytology, Tikhoretsky Ave. 4, 194064, St Petersburg, Russia.
Curr Genet. 2004 May;45(5):273-82. doi: 10.1007/s00294-004-0493-9. Epub 2004 Mar 13.
Heterokaryotic zygotes in yeast provide a unique possibility to study the survival and transmission of two genetically diverse nuclei in one cell. Using partial pedigree analysis, we show that various treatments used to change cytoplasmic hereditary determinants can essentially affect nuclear transmission in yeast heterokaryons. This includes choice of nucleus to enter the first bud and incidence of various classes of mother/daughter pairs demonstrating nuclear degradation patterns in heterokaryotic zygotes. These treatments include guanidine hydrochloride, a prion-curing agent, ethidium bromide, an agent causing elimination of mitochondrial DNA, and cytoplasm replacement by cytoduction, which leads to mtDNA replacement and transfer of some other cytoplasmically inherited determinants. The genetic and cytological evidence obtained favors prion involvement in nuclear transmission and suggests apoptotic features in nuclear degradation in yeast heterokaryotic zygotes.
酵母中的异核合子为研究一个细胞中两个基因不同的细胞核的存活和传递提供了独特的可能性。通过部分谱系分析，我们发现用于改变细胞质遗传决定因素的各种处理方法可本质上影响酵母异核体中的核传递。这包括进入第一个芽的细胞核的选择以及各类母/女对的发生率，这些母/女对展示了异核合子中的核降解模式。这些处理方法包括盐酸胍（一种朊病毒治愈剂）、溴化乙锭（一种导致线粒体DNA消除的试剂）以及通过细胞融合进行细胞质置换（这会导致线粒体DNA置换并转移一些其他细胞质遗传决定因素）。所获得的遗传和细胞学证据支持朊病毒参与核传递，并表明酵母异核合子中核降解具有凋亡特征。
