Conde J, Fink G R
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3651-5. doi: 10.1073/pnas.73.10.3651.
A mutant unable to fuse nuclei during mating has been isolated from standard wild-type Saccharomyces cerevisiae. Tetrad analysis of the mutation responsible for this defect (kar1-1) shows that it segregates as a single Mendelian factor. The defect kn kaf1-1 appears to be nuclear limited. Cytological and genetic evidence shows that in this mutant the events associated with zygote formation are normal until the point of nuclear fusion. The consequence of this defect is the formation of a multinucleate zygote which in subsequent divisions can segregate heterokaryons and haploid heterplasmons.
从标准野生型酿酒酵母中分离出了一种在交配过程中无法融合细胞核的突变体。对导致这种缺陷的突变（kar1-1）进行四分体分析表明，它作为一个单一的孟德尔因子进行分离。kar1-1缺陷似乎仅限于细胞核。细胞学和遗传学证据表明，在这个突变体中，与合子形成相关的事件在细胞核融合之前都是正常的。这种缺陷的结果是形成了一个多核合子，在随后的分裂中，它可以分离出异核体和单倍体异质体。
