以高锰酸钾为氧化剂的动力学分光光度法测定药物制剂中的水飞蓟素。
Kinetic spectrophotometric method for the determination of silymarin in pharmaceutical formulations using potassium permanganate as oxidant.
作者信息
Rahman N, Khan N A, Azmi S N H
机构信息
Analytical Chemistry Division, Department of Chemistry, Aligarh Muslim University, Aligarh (U.P.), India.
出版信息
Pharmazie. 2004 Feb;59(2):112-6.
A new simple and sensitive kinetic spectrophotometric method for the determination of silymarin in pure form and in pharmaceutical formulations is described. The method is based on the oxidation of the drug with potassium permanganate at pH 7.0 +/- 0.2. The reaction is followed spectrophotometrically by measuring the decrease in the absorbance at 530 nm. The calibration graph is linear in the range of 18-50 microg x m(-1). The method has been successfully applied to the determination of silymarin in pharmaceutical formulations. Statistical comparison of the results with the reference method shows excellent agreement and indicates no significant difference in accuracy and precision.
描述了一种用于测定纯品和药物制剂中水飞蓟素的新的简单灵敏的动力学分光光度法。该方法基于在pH 7.0±0.2条件下高锰酸钾对药物的氧化反应。通过测量530 nm处吸光度的降低，用分光光度法跟踪反应。校准曲线在18 - 50 μg·m⁻¹范围内呈线性。该方法已成功应用于药物制剂中水飞蓟素的测定。结果与参考方法的统计比较显示出极佳的一致性，表明在准确度和精密度方面无显著差异。
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