Omar Mahmoud A, Abdelmageed Osama H, Attia Tamer Z
Analytical Chemistry Department, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt.
Int J Anal Chem. 2009;2009:596379. doi: 10.1155/2009/596379. Epub 2009 Jun 21.
A simple, reliable, and sensitive kinetic spectrophotometric method was developed for determination of eight cephalosporin antibiotics, namely, Cefotaxime sodium, Cephapirin sodium, Cephradine dihydrate, Cephalexin monohydrate, Ceftazidime pentahydrate, Cefazoline sodium, Ceftriaxone sodium, and Cefuroxime sodium. The method depends on oxidation of each of studied drugs with alkaline potassium permanganate. The reaction is followed spectrophotometrically by measuring the rate of change of absorbance at 610 nm. The initial rate and fixed time (at 3 minutes) methods are utilized for construction of calibration graphs to determine the concentration of the studied drugs. The calibration graphs are linear in the concentration ranges 5-15 mug mL(-1) and 5-25 mug mL(-1) using the initial rate and fixed time methods, respectively. The results are validated statistically and checked through recovery studies. The method has been successfully applied for the determination of the studied cephalosporins in commercial dosage forms. Statistical comparisons of the results with the reference methods show the excellent agreement and indicate no significant difference in accuracy and precision.
建立了一种简单、可靠且灵敏的动力学分光光度法，用于测定8种头孢菌素抗生素，即头孢噻肟钠、头孢匹林钠、头孢拉定二水合物、头孢氨苄一水合物、头孢他啶五水合物、头孢唑林钠、头孢曲松钠和头孢呋辛钠。该方法基于所研究的每种药物与碱性高锰酸钾的氧化反应。通过在610 nm处测量吸光度的变化速率，采用分光光度法跟踪反应。利用初始速率法和固定时间法（3分钟时）绘制校准曲线，以确定所研究药物的浓度。分别采用初始速率法和固定时间法时，校准曲线在浓度范围5 - 15 μg mL⁻¹和5 - 25 μg mL⁻¹内呈线性。对结果进行了统计学验证，并通过回收率研究进行了检验。该方法已成功应用于商业剂型中所研究头孢菌素的测定。结果与参考方法的统计学比较显示出极好的一致性，表明在准确度和精密度方面无显著差异。
