Krishna B V S, Deshpande Asha R
Department of Microbiology, Karnatak Institute of Medical Sciences, Hubli.
Indian J Pathol Microbiol. 2003 Jul;46(3):517-20.
Stained blood film examination is a widely used technique for the diagnosis of malaria. Comparison of this technique was made with the QBC method, which is based on fluorescent staining of the blood cells and parasites. Of the 1435 blood samples studied, 57 (3.97%) samples were positive for malarial parasites by QBC method, while only 44 (3.07%) samples were positive by the blood film examination. Plasmodium vivax was detected in 27 (47.37%), P. falciparum in 26 (45.61%) and mixed infection of P. vivax and P. falciparum in 4 (7.02%) cases. Samples with low levels of parasitaemia (QBC grades 1+ and 2+) were often found to be negative by blood film examination. QBC method was easy to perform, had a higher sensitivity and could be interpreted rapidly, as compared to the Leishman stained blood film examination.
血涂片检查是一种广泛用于疟疾诊断的技术。将该技术与基于血细胞和寄生虫荧光染色的QBC方法进行了比较。在所研究的1435份血样中，QBC方法检测到57份（3.97%）疟原虫阳性样本，而血涂片检查仅检测到44份（3.07%）阳性样本。间日疟原虫在27例（47.37%）中被检测到，恶性疟原虫在26例（45.61%）中被检测到，4例（7.02%）为间日疟原虫和恶性疟原虫混合感染。疟原虫血症水平较低（QBC分级1+和2+）的样本通过血涂片检查常被发现为阴性。与利什曼染色血涂片检查相比，QBC方法操作简便，灵敏度更高，且能快速解读。
