Hemvani N, Chitnis D S, Dixit D S, Asolkar M V
Department Pathology, Choithram Hospital, Indore, India.
Indian J Pathol Microbiol. 1999 Apr;42(2):125-8.
The age old Romanowsky stained thick blood smear examination for malarial parasites may fail to reveal the low parasitaemia. The commercial 'QBC' like acridine orange stained capillary tube preparation has a limitation of precise species identification and the detection of extra-erythrocytic parasites. Hence, the present study was aimed to improve malarial parasite detection by using acridine orange to stain large blood drops in the form of wet coverglass mounts. The acridine orange stained blood wet mounts over 2420 suspected malaria cases from Indore city were examined under fluorescent microscope and the results compared with the Leishman's stained thick blood smears in a blind study. The positivity of malarial parasites reported by the modified acridine orange staining was 248 against 109 by Leishman's stained thick blood smears. The modified acridine orange stained method is simple, instant and more efficient, requires less scanning time and skill, allows scanning of larger blood volume (75 ul) at lower magnification and the morphological details at higher magnification helps to make the precise species identification.
用于检测疟原虫的传统罗曼诺夫斯基染色厚血涂片检查可能无法发现低疟原虫血症。类似“QBC”的吖啶橙染色毛细管制剂在商业上使用时，存在无法精确鉴定疟原虫种类以及检测不出红细胞外期疟原虫的局限性。因此，本研究旨在通过使用吖啶橙对湿盖玻片标本形式的大血滴进行染色，来改进疟原虫检测方法。在荧光显微镜下检查了来自印多尔市的2420例疑似疟疾病例的吖啶橙染色血液湿标本，并在一项盲法研究中将结果与利什曼染色厚血涂片进行比较。改良吖啶橙染色法报告的疟原虫阳性率为248例，而利什曼染色厚血涂片为109例。改良吖啶橙染色法简单、快速且更有效，所需的扫描时间和技能较少，能够在较低放大倍数下扫描更大体积的血液（75微升），且在较高放大倍数下的形态细节有助于精确鉴定疟原虫种类。
