次氮基三乙酸修饰的磁性纳米颗粒作为结合组氨酸标签蛋白的通用试剂。
Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins.
作者信息
Xu Chenjie, Xu Keming, Gu Hongwei, Zhong Xiaofen, Guo Zhihong, Zheng Rongkun, Zhang Xixiang, Xu Bing
机构信息
Department of Chemistry, Department of Physics, and Bioengineering Program, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
出版信息
J Am Chem Soc. 2004 Mar 24;126(11):3392-3. doi: 10.1021/ja031776d.
Using Nalpha,Nalpha-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt-NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.
通过使用Nα，Nα-双（羧甲基）赖氨酸与FePt磁性纳米颗粒反应，我们合成了FePt-NTA共轭物，它能固定Ni2+离子，并在低至0.5 pM的浓度下选择性结合组氨酸标记的蛋白质。这个简单的系统是现有蛋白质分离方案的有用替代方法，并且还可作为一种通用的蛋白质运输和锚定剂。
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